ArticleScientific reports2016
The Citrus transcription factor, CitERF13, regulates citric acid accumulation via a protein-protein interaction with the vacuolar proton pump, CitVHA-c4.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 79 citations in OpenAlex.
- Citric acid alters Arabidopsis root morphology and development through ROS-dependent and ROS-independent mechanisms.Plant physiology · 2026Article
- Molecular orchestration of malate and citrate metabolism: regulatory networks governing organic acid dynamics and fruit quality attributes.Horticulture research · 2026Article
- AP2 transcription factor CsAIL6 negatively regulates citric acid accumulation in citrus fruits by interacting with a WD40 protein CsAN11.Horticulture research · 2025Article
- Expression Analysis of Chlorophyll-Degradation-Related Genes inPlants (Basel, Switzerland) · 2025Article
- Comparative Transcriptome Analysis Reveals Potential Molecular Regulation of Organic Acid Metabolism During Fruit Development in Late-Maturing Hybrid Citrus Varieties.International journal of molecular sciences · 2025Article
- Influence of ZnSOInternational journal of molecular sciences · 2024Article
- The abscisic acid-responsive transcriptional regulatory module CsERF110-CsERF53 orchestrates citrus fruit coloration.Plant communications · 2024Article
- Dynamic Analysis of the Fruit Sugar-Acid Profile in a Fresh-Sweet Mutant and Wild Type in 'Shatangju' (Plants (Basel, Switzerland) · 2024Article
- Transcriptome Analysis Reveals Fruit Quality Formation inPlants (Basel, Switzerland) · 2023Article
- Regulation of a vacuolar proton-pumping P-ATPase MdPH5 by MdMYB73 and its role in malate accumulation and vacuolar acidification.aBIOTECH · 2023Article
- Transcriptome and Metabolome Reveal Sugar and Organic Acid Accumulation inPlants (Basel, Switzerland) · 2023Article
- A dramatic decline in fruit citrate induced by mutagenesis of a NAC transcription factor, AcNAC1.Plant biotechnology journal · 2023Article
- Article
- Transcriptome Analysis and VIGS Identification of Key Genes Regulating Citric Acid Metabolism in Citrus.Current issues in molecular biology · 2023Article
- Structural and Functional Diversity of Two ATP-Driven Plant Proton Pumps.International journal of molecular sciences · 2023Review
- Hormonal regulation ofFrontiers in plant science · 2023Article
- Heat production and volatile biosynthesis are linkedFrontiers in plant science · 2022Article
- Stable QTL for malate levels in ripe fruit and their transferability acrossHorticulture research · 2022Article
- The Interaction Between CitMYB52 and CitbHLH2 Negatively Regulates Citrate Accumulation by ActivatingFrontiers in plant science · 2022Article
- Transcriptome analysis of the pulp of citrus fruitlets suggests that domestication enhanced growth processes and reduced chemical defenses increasing palatability.Frontiers in plant science · 2022Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organic acids are essential to fruit flavor. The vacuolar H(+) transporting adenosine triphosphatase (V-ATPase) plays an important role in organic acid transport and accumulation. However, less is known of V-ATPase interacting proteins and their relationship with organic acid accumulation. The relationship between V-ATPase and citric acid was investigated, using the citrus tangerine varieties 'Ordinary Ponkan (OPK)' and an early maturing mutant 'Zaoshu Ponkan (ZPK)'. Five V-ATPase genes (CitVHA) were predicted as important to citric acid accumulation. Among the genes, CitVHA-c4 was observed, using a yeast two-hybrid screen, to interact at the protein level with an ethylene response factor, CitERF13. This was verified using bimolecular fluorescence complementation assays. A similar interaction was also observed between Arabidopsis AtERF017 (a CitERF13 homolog) and AtVHA-c4 (a CitVHA-c4 homolog). A synergistic effect on citric acid levels was observed between V-ATPase proteins and interacting ERFs when analyzed using transient over-expression in tobacco and Arabidopsis mutants. Furthermore, the transcript abundance of CitERF13 was concomitant with CitVHA-c4. CitERF13 or AtERF017 over-expression leads to significant citric acid accumulation. This accumulation was abolished in an AtVHA-c4 mutant background. ERF-VHA interactions appear to be involved in citric acid accumulation, which was observed in both citrus and Arabidopsis.
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