ArticleNMR in biomedicine2016
Metabolism of hyperpolarized [1-(13)C]pyruvate through alternate pathways in rat liver.
Article in NMR in biomedicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03480594 (Effect of Fatty Liver on TCA Cycle Flux and the Pentose Phosphate Pathway), which is not on this map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Effect of Fatty Liver on TCA Cycle Flux and the Pentose Phosphate Pathway (HP FFF)
Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- Hyperpolarized MRI in Oncology-A Review of Clinical Studies and Evaluation of Preclinical Liver Studies.NMR in biomedicine · 2026Review
- Disrupted metabolic flux balance between pyruvate dehydrogenase and pyruvate carboxylase in human fatty liver.Metabolism: clinical and experimental · 2025Article
- The neglected PCK1/glucagon (inter)action in nutrient homeostasis beyond gluconeogenesis: Disease pathogenesis and treatment.Molecular metabolism · 2025Review
- The mitochondrial dicarboxylate carrier mediates in vivo hepatic gluconeogenesis.bioRxiv : the preprint server for biology · 2024Article
- Hyperpolarized [2-ACS sensors · 2024Article
- Observing exocrine pancreas metabolism using a novel pancreas perfusion technique in combination with hyperpolarized [1-Magnetic resonance in chemistry : MRC · 2023Article
- Oxidation of hyperpolarized [1-NMR in biomedicine · 2023Article
- Magnetic Resonance Imaging and Spectroscopy Methods to Study Hepatic Glucose Metabolism and Their Applications in the Healthy and Diabetic Liver.Metabolites · 2022Review
- DetectingFrontiers in physiology · 2022Article
- Regulation of Metabolism by Mitochondrial MUL1 E3 Ubiquitin Ligase.Frontiers in cell and developmental biology · 2022Article
- Hyperpolarized [1-Experimental physiology · 2021Article
- Co-Polarized [1-ACS sensors · 2021Article
- Article
- Hyperpolarized Dihydroxyacetone Is a Sensitive Probe of Hepatic Gluconeogenic State.Metabolites · 2021Article
- In Vivo Assessment of Metabolic Abnormality in Alport Syndrome Using Hyperpolarized [1-Metabolites · 2021Article
- Comprehensive Literature Review of Hyperpolarized Carbon-13 MRI: The Road to Clinical Application.Metabolites · 2021Review
- Assessment of hepatic pyruvate carboxylase activity using hyperpolarized [1-Magnetic resonance in medicine · 2021Article
- The use of hyperpolarisedBritish journal of cancer · 2021Review
- A Comprehensive Proteome and Acetyl-Proteome Atlas Reveals Molecular Mechanisms Adapting to the Physiological Changes From Pre-laying to Peak-Laying Stage in Liver of Hens (Frontiers in veterinary science · 2021Article
- Changes of Coenzyme A and Acetyl-Coenzyme A Concentrations in Rats after a Single-Dose Intraperitoneal Injection of Hepatotoxic Thioacetamide Are Not Consistent with Rapid Recovery.International journal of molecular sciences · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The source of hyperpolarized (HP) [(13)C]bicarbonate in the liver during metabolism of HP [1-(13)C]pyruvate is uncertain and likely changes with physiology. Multiple processes including decarboxylation through pyruvate dehydrogenase or pyruvate carboxylase followed by subsequent decarboxylation via phosphoenolpyruvate carboxykinase (gluconeogenesis) could play a role. Here we tested which metabolic fate of pyruvate contributed to the appearance of HP [(13)C]bicarbonate during metabolism of HP [1-(13)C]pyruvate by the liver in rats after 21 h of fasting compared to rats with free access to food. The (13)C NMR of HP [(13)C]bicarbonate was observed in the liver of fed rats, but not in fasted rats where pyruvate carboxylation and gluconeogenesis was active. To further explore the relative fluxes through pyruvate carboxylase versus pyruvate dehydrogenase in the liver under typical conditions of hyperpolarization studies, separate parallel experiments were performed with rats given non-hyperpolarized [2,3-(13)C]pyruvate. (13)C NMR analysis of glutamate isolated from the liver of rats revealed that flux from injected pyruvate through pyruvate dehydrogenase was dominant under fed conditions whereas flux through pyruvate carboxylase dominated under fasted conditions. The NMR signal of HP [(13)C]bicarbonate does not parallel pyruvate carboxylase activity followed by subsequent decarboxylation reaction leading to glucose production. In the liver of healthy well-fed rats, the appearance of HP [(13)C]bicarbonate exclusively reflects decarboxylation of HP [1-(13)C]pyruvate via pyruvate dehydrogenase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.