ArticleAnalytical chemistry2016
Investigation of Reagent Delivery Formats in a Multivalent Malaria Sandwich Immunoassay and Implications for Assay Performance.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Review
- Lateral flow assay sensitivity and signal enhancement via laser µ-machined constrains in nitrocellulose membrane.Scientific reports · 2024Article
- Glycosylated gold nanoparticles in point of care diagnostics: from aggregation to lateral flow.Chemical Society reviews · 2022Review
- Characterization of wax valving and μPIV analysis of microscale flow in paper-fluidic devices for improved modeling and design.Lab on a chip · 2022Article
- Automated liquid handling robot for rapid lateral flow assay development.Analytical and bioanalytical chemistry · 2022Article
- Rational design and characterization of a lateral flow assay for canine C-reactive protein in wound exudate.Talanta · 2020Article
- Unraveling the Hook Effect: A Comprehensive Study of High Antigen Concentration Effects in Sandwich Lateral Flow Immunoassays.Analytical chemistry · 2020Article
- Lateral flow immunoassay for 5-hydroxyflunixin based on near-infrared fluorescence molecule as an alternative label to gold nanoparticles.Mikrochimica acta · 2020Article
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Analytical Tools to Improve Optimization Procedures for Lateral Flow Assays.Diagnostics (Basel, Switzerland) · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Conventional lateral flow tests (LFTs), the current standard bioassay format used in low-resource point-of-care (POC) settings, have limitations that have held back their application in the testing of low concentration analytes requiring high sensitivity and low limits of detection. LFTs use a premix format for a rapid one-step delivery of premixed sample and labeled antibody to the detection region. We have compared the signal characteristics of two types of reagent delivery formats in a model system of a sandwich immunoassay for malarial protein detection. The premix format produced a uniform binding profile within the detection region. In contrast, decoupling the delivery of sample and labeled antibody to the detection region in a sequential format produced a nonuniform binding profile in which the majority of the signal was localized to the upstream edge of the detection region. The assay response was characterized in both the sequential and premix formats. The sequential format had a 4- to 10-fold lower limit of detection than the premix format, depending on assay conjugate concentration. A mathematical model of the assay quantitatively reproduced the experimental binding profiles for a set of rate constants that were consistent with surface plasmon resonance measurements and absorbance measurements of the experimental multivalent malaria system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.