ArticleDiagnostics (Basel, Switzerland)2013
Performance Evaluation of Fast Microfluidic Thermal Lysis of Bacteria for Diagnostic Sample Preparation.
Article in Diagnostics (Basel, Switzerland), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Direct quantification of waterborne viruses via high-temperature and high-pressure treatment: a simplified nucleic acid extraction-free approach.Scientific reports · 2026Article
- Thermostable Bacterial Esterases From Lipase Family 1.5 Degrade Compostable Polyesters PBAT and PBSA.MicrobiologyOpen · 2025Article
- Advanced microfluidic systems with temperature modulation for biological applications.Biomicrofluidics · 2025Article
- A traveling surface acoustic wave-based micropiezoactuator: A tool for additive- and label-free cell lysis.Biomicrofluidics · 2024Article
- Development of advanced control material for reverse transcription-mediated bacterial nucleic acid amplification tests.Journal of clinical microbiology · 2024Article
- Evaluation of cell disruption methods for protein and coenzyme Q10 quantification in purple non-sulfur bacteria.Frontiers in microbiology · 2024Article
- Highly Efficient On-Chip Photothermal Cell Lysis for Nucleic Acid Extraction Using Localized Plasmonic Heating of Strongly Absorbing Au Nanoislands.ACS applied materials & interfaces · 2023Article
- Article
- Review of Microfluidic Methods for Cellular Lysis.Micromachines · 2021Review
- Microfluidic enrichment of bacteria coupled to contact-free lysis on a magnetic polymer surface for downstream molecular detection.Biomicrofluidics · 2020Article
- Optical Temperature Control Unit and Convolutional Neural Network for Colorimetric Detection of Loop-Mediated Isothermal Amplification on a Lab-On-A-Disc Platform.Sensors (Basel, Switzerland) · 2019Article
- Particle Diffusometry: An Optical Detection Method for Vibrio cholerae Presence in Environmental Water Samples.Scientific reports · 2019Article
- Towards Multiplex Molecular Diagnosis-A Review of Microfluidic Genomics Technologies.Micromachines · 2017Review
- Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification.Scientific reports · 2017Article
- A Mutant Isoform of ObgE Causes Cell Death by Interfering with Cell Division.Frontiers in microbiology · 2017Article
- Time-resolved, single-cell analysis of induced and programmed cell death via non-invasive propidium iodide and counterstain perfusion.Scientific reports · 2016Article
- A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice.Journal of visualized experiments : JoVE · 2015Article
- Paper-based molecular diagnostic forRSC advances · 2014Article
- Use of surface enhanced blocking (SEB) electrodes for microbial cell lysis in flow-through devices.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Development of new diagnostic platforms that incorporate lab-on-a-chip technologies for portable assays is driving the need for rapid, simple, low cost methods to prepare samples for downstream processing or detection. An important component of the sample preparation process is cell lysis. In this work, a simple microfluidic thermal lysis device is used to quickly release intracellular nucleic acids and proteins without the need for additional reagents or beads used in traditional chemical or mechanical methods (e.g., chaotropic salts or bead beating). On-chip lysis is demonstrated in a multi-turn serpentine microchannel with external temperature control via an attached resistive heater. Lysis was confirmed for Escherichia coli by fluorescent viability assay, release of ATP measured with bioluminescent assay, release of DNA measured by fluorometry and qPCR, as well as bacterial culture. Results comparable to standard lysis techniques were achievable at temperatures greater than 65 °C and heating durations between 1 and 60 s.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.