ArticleAngewandte Chemie (International ed. in English)2016
Modified Nucleotides for Discrimination between Cytosine and the Epigenetic Marker 5-Methylcytosine.
Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Cytosine base modifications regulate DNA duplex stability and metabolism.Nucleic acids research · 2021Article
- Review
- Article
- Snapshots of a modified nucleotide moving through the confines of a DNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Copper carbenes alkylate guanine chemoselectively through a substrate directed reaction.Chemical science · 2017Article
- Selective detection of N6-methyladenine in DNAChemical science · 2017Article
- 5-methylcytosine-sensitive variants of Thermococcus kodakaraensis DNA polymerase.Nucleic acids research · 2016Article
- 6-Substituted 2-Aminopurine-2'-deoxyribonucleoside 5'-Triphosphates that Trace Cytosine Methylation.Chembiochem : a European journal of chemical biology · 2016Article
- Modified Nucleotides for Discrimination between Cytosine and the Epigenetic Marker 5-Methylcytosine.Angewandte Chemie (International ed. in English) · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
5-Methyl-2'-deoxycytosine, the most common epigenetic marker of DNA in eukaryotic cells, plays a key role in gene regulation and affects various cellular processes such as development and carcinogenesis. Therefore, the detection of 5mC can serve as an important biomarker for diagnostics. Here we describe that modified dGTP analogues as well as modified primers are able to sense the presence or absence of a single methylation of C, even though this modification does not interfere directly with Watson-Crick nucleobase pairing. By screening several modified nucleotide scaffolds, O(6)-modified 2'-deoxyguanosine analogues were identified as discriminating between C and 5mC. These modified nucleotides might find application in site-specific 5mC detection, for example, through real-time PCR approaches.
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Registered trials
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