Evidence map›Paper›PMID 26834992›Full record

ArticleF1000Research2015

MinION Analysis and Reference Consortium: Phase 1 data release and analysis.

Camilla L C Ip, Matthew Loose, John R Tyson, Mariateresa de Cesare, Bonnie L Brown, Miten Jain, Richard M Leggett, David A Eccles, Vadim Zalunin, John M Urban and 14 more

Abstract read
In one paragraph

Article in F1000Research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 160 papers.

0numbers the graph read from it
0cells of the map it votes in
160citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

160 citing papers in PubMed.

  1. Article
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  5. Review
  6. Nanopore sequencing of the Tunisian gut microbiome: effect of the DNA extraction methods.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  7. Review
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  9. Article
  10. Infection and immunity · 2025
    Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
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  18. Article
  19. Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025
    Review
  20. Article

100 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Camilla L C Ip *Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.
Matthew Loose *School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham, UK.
John R Tyson *Michael Smith Laboratories and Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
Mariateresa de Cesare *Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.
Bonnie L Brown *Virginia Commonwealth University, Richmond, VA, USA.
Miten Jain *University of California, Santa Cruz, Santa Cruz, CA, USA.
Richard M Leggett *The Genome Analysis Centre, Norwich Research Park, Norwich, UK.
David A EcclesMalaghan Institute of Medical Research, Wellington, New Zealand.
Vadim ZaluninEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, UK.
John M UrbanDivision of Biology and Medicine, Brown University, Providence, RI, USA.
Paolo PiazzaWellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.
Rory J BowdenWellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.
Benedict PatenUniversity of California, Santa Cruz, Santa Cruz, CA, USA.
Solomon MwaigwisyaNorwich Medical School, University of East Anglia, Norwich, UK.
Elizabeth M BattyWellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.
Jared T SimpsonInformatics and Biocomputing, Ontario Institute for Cancer Research, ON, Canada.
Terrance P SnutchMichael Smith Laboratories and Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, Canada.
Ewan Birney *European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, UK.
David Buck *Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.
Sara Goodwin *Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
Hans J Jansen *ZF-screens B.V., Leiden, Netherlands.
Justin O'Grady *Norwich Medical School, University of East Anglia, Norwich, UK.
Hugh E Olsen *University of California, Santa Cruz, Santa Cruz, CA, USA.
MinION Analysis and Reference Consortium

Funding

Single-Cell Biology Shared ResourceP30CA045508 · NCI · COLD SPRING HARBOR LABORATORY · PI John Edward Moses · 1987 to 2026
$118.9M
Training SubprojectU54HG007990 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI HAUSSLER, DAVID H, PATTERSON, DAVID · 2014 to 2018
$14.8M
Computational Methods for Genome Assembly, Transcript Assembly, and Variant DiscoveryR01HG006677 · NHGRI · JOHNS HOPKINS UNIVERSITY · PI SALZBERG, STEVEN L. · 2011 to 2025
$10.7M
Optimization of Nanopore Genomic DNA SequencingR01HG007827 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI AKESON, MARK A · 2014 to 2016
$2.2M
Biotechnology and Biological Sciences Research Council BB/M020061/1NCI NIH HHS P30 CA045508NHGRI NIH HHS R01 HG006677NHGRI NIH HHS R01 HG007827NHGRI NIH HHS U54 HG007990Wellcome Trust
6 · The paper itself

Abstract

The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. The MinION™ Access Programme (MAP) was initiated by Oxford Nanopore Technologies™ in April 2014, giving public access to their USB-attached miniature sequencing device. The MinION Analysis and Reference Consortium (MARC) was formed by a subset of MAP participants, with the aim of evaluating and providing standard protocols and reference data to the community. Envisaged as a multi-phased project, this study provides the global community with the Phase 1 data from MARC, where the reproducibility of the performance of the MinION was evaluated at multiple sites. Five laboratories on two continents generated data using a control strain of Escherichia coli K-12, preparing and sequencing samples according to a revised ONT protocol. Here, we provide the details of the protocol used, along with a preliminary analysis of the characteristics of typical runs including the consistency, rate, volume and quality of data produced. Further analysis of the Phase 1 data presented here, and additional experiments in Phase 2 of E. coli from MARC are already underway to identify ways to improve and enhance MinION performance.

Indexed as

data releaselong readsmarginAlignMinIONminoTourNanoOKnanopore sequencingthird-generation sequencing

Identifiers

PMID26834992
PMCPMC4722697

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.