ArticleFrontiers in plant science2015
Validating DNA Polymorphisms Using KASP Assay in Prairie Cordgrass (Spartina pectinata Link) Populations in the U.S.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
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- Genetic mapping of fertility restoration (Rf) genes and development-validation of linked SNPs for AJournal of applied genetics · 2026Article
- Factors affecting the genetic diversity ofFrontiers in plant science · 2026Review
- Development of KASP molecular markers and construction of fingerprinting for cowpea (Vigna unguiculata (L.) Walp.) based on ddRAD-Seq.PloS one · 2026Article
- Decoding plant responses to waterlogging: from stress signals to molecular mechanisms and their future implications.Plant molecular biology · 2025Review
- Evaluating the Effects ofInsects · 2024Article
- Antisense RNA (asRNA) technology: the concept and applications in crop improvement and sustainable agriculture.Molecular biology reports · 2023Review
- Kompetitive Allele Specific PCR Genotyping of 89 SNPs in Romanian Spotted and Romanian Brown Cattle Breeds and Their Association with Clinical Mastitis.Animals : an open access journal from MDPI · 2023Article
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- Quantitative trait loci (QTL) analysis of leaf related traits in spinach (Spinacia oleracea L.).BMC plant biology · 2021Article
- Development of genic KASP SNP markers from RNA-Seq data for map-based cloning and marker-assisted selection in maize.BMC plant biology · 2021Article
- Introgression of Maize Lethal Necrosis Resistance Quantitative Trait Loci Into Susceptible Maize Populations and Validation of the Resistance Under Field Conditions in Naivasha, Kenya.Frontiers in plant science · 2021Article
- Single nucleotide polymorphisms associated with postoperative inadequate analgesia after single-port VATS in Chinese population.BMC anesthesiology · 2020Article
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- Fine mapping and candidate gene screening of the downy mildew resistance gene RPF1 in Spinach.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
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- Advantages of Amplifluor-like SNP markers over KASP in plant genotyping.BMC plant biology · 2017Article
- Meiotic pairing as an indicator of genome composition in polyploid prairie cordgrass (Spartina pectinata Link).Genetica · 2017Article
- An RNA Sequencing Transcriptome Analysis of Grasspea (Frontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single nucleotide polymorphisms (SNPs) are one of the most abundant DNA variants found in plant genomes and are highly efficient when comparing genome and transcriptome sequences. SNP marker analysis can be used to analyze genetic diversity, create genetic maps, and utilize marker-assisted selection breeding in many crop species. In order to utilize these technologies, one must first identify and validate putative SNPs. In this study, 121 putative SNPs, developed from a nuclear transcriptome of prairie cordgrass (Spartina pectinata Link), were analyzed using KASP technology in order to validate the SNPs. Fifty-nine SNPs were validated using a core collection of 38 natural populations and a phylogenetic tree was created with one main clade. Samples from the same population tended to cluster in the same location on the tree. Polymorphisms were identified within 52.6% of the populations, split evenly between the tetraploid and octoploid cytotypes. Twelve selected SNP markers were used to assess the fidelity of tetraploid crosses of prairie cordgrass and their resulting F2population. These markers were able to distinguish true crosses and selfs. This study provides insight into the genomic structure of prairie cordgrass, but further analysis must be done on other cytotypes to fully understand the structure of this species. This study validates putative SNPs and confirms the potential usefulness of SNP marker technology in future breeding programs of this species.
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