Evidence map›Paper›PMID 26834087›Full record

ArticleProteomics2016

Defining the proteome of human iris, ciliary body, retinal pigment epithelium, and choroid.

Pingbo Zhang, David Kirby, Craig Dufresne, Yan Chen, Randi Turner, Sara Ferri, Deepak P Edward, Jennifer E Van Eyk, Richard D Semba

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
5.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 34 citations in OpenAlex.

  1. Review
  2. Activity and Expression of Carboxylesterases and Arylacetamide Deacetylase in Human Ocular Tissues.Drug metabolism and disposition: the biological fate of chemicals · 2022
    Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Dissection of Human Retina and RPE-Choroid for Proteomic Analysis.Journal of visualized experiments : JoVE · 2017
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Proteomics of Tear in Inactive Thyroid-Associated Ophthalmopathy.Acta endocrinologica (Bucharest, Romania : 2005)
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Pingbo ZhangDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
David KirbyDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Craig DufresneThermo Fisher Scientific, West Palm Beach, FL, USA.
Yan ChenDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Randi TurnerDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Sara FerriDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Deepak P EdwardDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jennifer E Van EykAdvanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Richard D SembaDepartment of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Johns Hopkins University · USJohns Hopkins Medicine · USCedars-Sinai Smidt Heart Institute · USKing Khaled Eye Specialist Hospital · SAThermo Fisher Scientific (United States) · US

Funding

Oxidative stress and the pathogenesis of sarcopenia and disability in older womenR01AG027012 · NIA · JOHNS HOPKINS UNIVERSITY · PI SEMBA, RICHARD D · 2005 to 2018
$3.6M
Systemic complement and age-related macular degeneration.R01EY024596 · NEI · JOHNS HOPKINS UNIVERSITY · PI SEMBA, RICHARD D · 2014 to 2016
$1.6M
Klotho and the pathogenesis of cardiovascular diseaseR01HL111271 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI SEMBA, RICHARD D · 2012 to 2015
$1.5M
NEI NIH HHS R01 EY024596NHLBI NIH HHS R01 HL111271NIA NIH HHS R01 AG027012
6 · The paper itself

Abstract

The iris is a fine structure that controls the amount of light that enters the eye. The ciliary body controls the shape of the lens and produces aqueous humor. The retinal pigment epithelium and choroid (RPE/choroid) are essential in supporting the retina and absorbing light energy that enters the eye. Proteins were extracted from iris, ciliary body, and RPE/choroid tissues of eyes from five individuals and fractionated using SDS-PAGE. After in-gel digestion, peptides were analyzed using LC-MS/MS on an Orbitrap Elite mass spectrometer. In iris, ciliary body, and RPE/choroid, we identified 2959, 2867, and 2755 nonredundant proteins with peptide and protein false-positive rates of <0.1% and <1%, respectively. Forty-three unambiguous protein isoforms were identified in iris, ciliary body, and RPE/choroid. Four "missing proteins" were identified in ciliary body based on ≥2 proteotypic peptides. The mass spectrometric proteome database of the human iris, ciliary body, and RPE/choroid may serve as a valuable resource for future investigations of the eye in health and disease. The MS proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD001424 and PXD002194.

Indexed as

AgedElectrophoresis, Gel, Two-DimensionalEye ProteinsFemaleHumansMaleMiddle AgedProteomeProteomicsRetinal Pigment EpitheliumUveaEye ProteinsProteomeBiomedicineChoroidCiliary bodyEyeIrisRetinal pigment epithelium

Identifiers

PMID26834087
PMCPMC4833206
OpenAlexW2263016555

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.