ArticleThe Journal of cell biology2016
Unattached kinetochores rather than intrakinetochore tension arrest mitosis in taxol-treated cells.
Article in The Journal of cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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Who cites it
43 citing papers in PubMed.
- Mitotic error correction and the spindle assembly checkpoint: a tension-filled relationship.Cell cycle (Georgetown, Tex.) · 2026Review
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Review
- Direct observation of interdependent and hierarchical kinetochore assembly on individual centromeres.Nucleic acids research · 2025Article
- One step 4× and 12× 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures.The Journal of cell biology · 2025Article
- Principles and dynamics of spindle assembly checkpoint signalling.Nature reviews. Molecular cell biology · 2023Review
- Preparation of Mitotic Cells for Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The Four Causes: The Functional Architecture of Centromeres and Kinetochores.Annual review of genetics · 2022Review
- Kinetochore assembly throughout the cell cycle.Seminars in cell & developmental biology · 2021Article
- Chromosomal instability: Stretching the role of checkpoint silencing.Current biology : CB · 2021Article
- Trypanosome KKIP1 Dynamically Links the Inner Kinetochore to a Kinetoplastid Outer Kinetochore Complex.Frontiers in cellular and infection microbiology · 2021Article
- Dynamics of kinetochore structure and its regulations during mitotic progression.Cellular and molecular life sciences : CMLS · 2020Review
- Effects of malleable kinetochore morphology on measurements of intrakinetochore tension.Open biology · 2020Article
- The centromere comes into focus: from CENP-A nucleosomes to kinetochore connections with the spindle.Open biology · 2020Review
- Ensemble-Level Organization of Human Kinetochores and Evidence for Distinct Tension and Attachment Sensors.Cell reports · 2020Article
- A comparative analysis of methods to measure kinetochore-microtubule attachment stability.Methods in cell biology · 2020Article
- Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner.The Journal of cell biology · 2019Article
- The mammalian kinetochore-microtubule interface: robust mechanics and computation with many microtubules.Current opinion in cell biology · 2019Review
- A tension-independent mechanism reduces Aurora B-mediated phosphorylation upon microtubule capture by CENP-E at the kinetochore.Cell cycle (Georgetown, Tex.) · 2019Article
- Checkpoint Proteins Bub1 and Bub3 Delay Anaphase Onset in Response to Low Tension Independent of Microtubule-Kinetochore Detachment.Cell reports · 2019Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Kinetochores attach chromosomes to the spindle microtubules and signal the spindle assembly checkpoint to delay mitotic exit until all chromosomes are attached. Light microscopy approaches aimed to indirectly determine distances between various proteins within the kinetochore (termed Delta) suggest that kinetochores become stretched by spindle forces and compact elastically when the force is suppressed. Low Delta is believed to arrest mitotic progression in taxol-treated cells. However, the structural basis of Delta remains unknown. By integrating same-kinetochore light microscopy and electron microscopy, we demonstrate that the value of Delta is affected by the variability in the shape and size of outer kinetochore domains. The outer kinetochore compacts when spindle forces are maximal during metaphase. When the forces are weakened by taxol treatment, the outer kinetochore expands radially and some kinetochores completely lose microtubule attachment, a condition known to arrest mitotic progression. These observations offer an alternative interpretation of intrakinetochore tension and question whether Delta plays a direct role in the control of mitotic progression.
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