ArticleFluids and barriers of the CNS2016
Modeling immune functions of the mouse blood-cerebrospinal fluid barrier in vitro: primary rather than immortalized mouse choroid plexus epithelial cells are suited to study immune cell migration across this brain barrier.
Article in Fluids and barriers of the CNS, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Primary cell and tissue cultures of human choroid plexus epithelial cells.Frontiers in cellular neuroscience · 2026Article
- Paclitaxel triggers molecular and cellular changes in the choroid plexus.Frontiers in pain research (Lausanne, Switzerland) · 2024Article
- The choroid plexus acts as an immune cell reservoir and brain entry site in experimental autoimmune encephalomyelitis.Fluids and barriers of the CNS · 2023Article
- Characterization of TRPV4-mediated signaling pathways in an optimized human choroid plexus epithelial cell line.American journal of physiology. Cell physiology · 2022Article
- Different Involvement of Vimentin during Invasion byInternational journal of molecular sciences · 2022Article
- Immune response and pathogen invasion at the choroid plexus in the onset of cerebral toxoplasmosis.Journal of neuroinflammation · 2022Article
- Brain endothelial tricellular junctions as novel sites for T cell diapedesis across the blood-brain barrier.Journal of cell science · 2021Article
- Central Nervous System Barriers Impact Distribution and Expression of iNOS and Arginase-1 in Infiltrating Macrophages During Neuroinflammation.Frontiers in immunology · 2021Article
- A narrative review of central nervous system involvement in acute leukemias.Annals of translational medicine · 2021Review
- Advancing brain barriers RNA sequencing: guidelines from experimental design to publication.Fluids and barriers of the CNS · 2020Review
- Zika virus infects pericytes in the choroid plexus and enters the central nervous system through the blood-cerebrospinal fluid barrier.PLoS pathogens · 2020Article
- In vitro modeling of blood-brain barrier and interface functions in neuroimmune communication.Fluids and barriers of the CNS · 2020Review
- Human CD4Fluids and barriers of the CNS · 2020Article
- Dwellers and Trespassers: Mononuclear Phagocytes at the Borders of the Central Nervous System.Frontiers in immunology · 2020Review
- Claudin-3-deficient C57BL/6J mice display intact brain barriers.Scientific reports · 2019Article
- Accurate, strong, and stable reporting of choroid plexus epithelial cells in transgenic mice using a human transthyretin BAC.Fluids and barriers of the CNS · 2018Article
- The Role of the Central Nervous System Microenvironment in Pediatric Acute Lymphoblastic Leukemia.Frontiers in pediatrics · 2017Review
- Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?Journal of tissue engineeringReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe blood-cerebrospinal fluid barrier (BCSFB) established by the choroid plexus (CP) epithelium has been recognized as a potential entry site of immune cells into the central nervous system during immunosurveillance and neuroinflammation. The location of the choroid plexus impedes in vivo analysis of immune cell trafficking across the BCSFB. Thus, research on cellular and molecular mechanisms of immune cell migration across the BCSFB is largely limited to in vitro models. In addition to forming contact-inhibited epithelial monolayers that express adhesion molecules, the optimal in vitro model must establish a tight permeability barrier as this influences immune cell diapedesis.
methodsWe compared cell line models of the mouse BCSFB derived from the Immortomouse(®) and the ECPC4 line to primary mouse choroid plexus epithelial cell (pmCPEC) cultures for their ability to establish differentiated and tight in vitro models of the BCSFB.
resultsWe found that inducible cell line models established from the Immortomouse(®) or the ECPC4 tumor cell line did not express characteristic epithelial proteins such as cytokeratin and E-cadherin and failed to reproducibly establish contact-inhibited epithelial monolayers that formed a tight permeability barrier. In contrast, cultures of highly-purified pmCPECs expressed cytokeratin and displayed mature BCSFB characteristic junctional complexes as visualized by the junctional localization of E-cadherin, β-catenin and claudins-1, -2, -3 and -11. pmCPECs formed a tight barrier with low permeability and high electrical resistance. When grown in inverted filter cultures, pmCPECs were suitable to study T cell migration from the basolateral to the apical side of the BCSFB, thus correctly modelling in vivo migration of immune cells from the blood to the CSF.
conclusionsOur study excludes inducible and tumor cell line mouse models as suitable to study immune functions of the BCSFB in vitro. Rather, we introduce here an in vitro inverted filter model of the primary mouse BCSFB suited to study the cellular and molecular mechanisms mediating immune cell migration across the BCSFB during immunosurveillance and neuroinflammation.
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