ArticleScientific reports2016
Lectin binding of human sperm associates with DEFB126 mutation and serves as a potential biomarker for subfertility.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
- Global characterization of mouse testis O-glycoproteome landscape during spermatogenesis.Nature communications · 2025Article
- Beta-defensin 126 (DEFB126) localization and expression in the sperms and male reproductive tract of the dromedary camel (Open veterinary journal · 2025Article
- Deciphering disease through glycan codes: leveraging lectin microarrays for clinical insights.Acta biochimica et biophysica Sinica · 2024Review
- Glycans in spent embryo culture medium are related to the implantation ability of blastocysts.Heliyon · 2023Article
- Defensins: defenders of human reproductive health.Human reproduction update · 2023Review
- Buffalo sperm surface proteome profiling reveals an intricate relationship between innate immunity and reproduction.BMC genomics · 2021Article
- Application of Lectin Microarrays for Biomarker Discovery.ChemistryOpen · 2020Review
- A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.Frontiers in immunology · 2020Article
- Signal peptide peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins.EMBO reports · 2019Article
- Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker.Clinical proteomics · 2018Article
- A standardized method for lectin microarray-based tissue glycome mapping.Scientific reports · 2017Article
- A Human Lectin Microarray for Sperm Surface Glycosylation Analysis.Molecular & cellular proteomics : MCP · 2016Article
- DEFB126 2-nt Deletion (rs11467417) as a Potential Risk Factor for Chlamydia Trachomatis Infection and Subsequent Infertility in Iranian Men.Journal of reproduction & infertilityArticle
- Sperm-specific protein ACTL7A as a biomarker for fertilization outcomes of assisted reproductive technology.Asian journal of andrologyArticle
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Authors and funding
18 authors at 6 institutions in 1 country.
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Abstract
Coating on the sperm surface, glycocalyx, plays a key role in sperm motility, maturation and fertilization. A comprehensive profile of sperm surface glycans will greatly facilitate both basic researches and clinical studies. Because of the capability of recognizing different glycan moieties, lectins are widely used in glycobiology. However, lacking high-throughput technology, limited lectins have been reported for analyzing the glycan of human sperm. In this study, we employed a lectin microarray for profiling the surface glycans of human sperm, on which 54 out of 91 lectins showed positive binding. Based on this technique, we compared lectin binding profiling of sperm with homozygous DEFB126 mutation (del/del) with that of wild type (wt/wt). DEFB126 was reported to contribute to the sialylation on sperm surface and its homozygous mutation was related to male subfertility. Six lectins (Jacalin/AIA, GHA, ACL, MPL, VVL and ABA) were found to develop lower binding affinity to sperm with del/del. Further validation showed that these lectins, especially ABA and MPL, can be potential biomarkers for clinical diagnosis of subfertility due to the mutation of DEFB126. Our research provides insight into the detection of some unexplained male subfertility, and the lectin microarray is generally applicable for infertility/subfertility sperm biomarker discovery.
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