Evidence map›Paper›PMID 26832678›Full record

ArticleMolecular imaging and biology2016

Detection of Klebsiella. Pneumoniae Infection with an Antisense Oligomer Against its Ribosomal RNA.

Ling Chen, Dengfeng Cheng, Guozheng Liu, Shuping Dou, Yuzhen Wang, Xinrong Liu, Yuxia Liu, Mary Rusckowski

Abstract read
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In one paragraph

Article in Molecular imaging and biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Ling ChenDivision of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
Dengfeng ChengDepartment of Nuclear Medicine, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Guozheng LiuDivision of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
Shuping DouDivision of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
Yuzhen WangDivision of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA.
Xinrong LiuImmunoGen Inc, Waltham, MA, 02451, USA.
Yuxia LiuShanghai Institute of Applied Physics, Chinese Academy of Sciences, , Shanghai, 201800, China.
Mary RusckowskiDivision of Nuclear Medicine, Department of Radiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA, 01655, USA. mary.rusckowski@umassmed.edu.
University of Massachusetts Chan Medical School · USChinese Academy of Sciences · CNFudan University · CNImmunoGen (United States) · US

Funding

Bacterial Specific Oligomers for Infection Detection Through ImagingR21AI070857 · NIAID · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI RUSCKOWSKI, MARY · 2007 to 2008
$434k
NIAID NIH HHS R21 AI070857
6 · The paper itself

Abstract

purposePreviously, we demonstrated specific accumulation into bacteria of a 12-mer phosphorodiamidate morpholino (MORF) oligomer complementary to a ribosomal RNA (rRNA) segment found in all bacteria using the universal probe called Eub338 (Eub). Here, two MORF oligomers Eco and Kpn with sequences specific to the rRNA of Escherichia coli (Eco) and Klebsiella pneumoniae (Kpn) were investigated along with Eub and control (nonEub). PROCEDURES: To determine bacterial rRNA binding, oligomers were tagged with Alexa Fluor 633 (AF633) for fluorescence in situ hybridization (FISH) and fluorescence microscopy, and radiolabeled with technetium-99m (Tc-99m) for biodistribution and SPECT imaging in infected mice.

resultsBy both FISH and fluorescence microscopy, Eub showed a positive signal in both E. coli and K. pneumoniae as expected, and Kpn showed significantly higher accumulation in K. pneumoniae with near background in E. coli (p < 0.01). Conversely, Eco was positive in both E. coli and K. pneumoniae, hence nonspecific. As determined by biodistribution, the accumulation of [(99m)Tc]Kpn was higher in the thigh infected with live K. pneumoniae than with live E. coli (p = 0.05), and significantly higher than with heat-killed K. pneumoniae (p = 0.02) in the target thigh. By SPECT imaging, the accumulation of [(99m)Tc]Kpn was obviously higher in its specific target of K. pneumoniae compared to an E. coli infected thigh.

conclusionsKpn complementary to the rRNA of K. pneumoniae, labeled with Tc-99m or AF633, demonstrated specific binding to fixed and live K. pneumoniae in culture and in infected mice such that Tc-99m-labeled Kpn as the MORF oligomer may be useful for K. pneumoniae infection detection through imaging.

Indexed as

AnimalsIn Situ Hybridization, FluorescenceKlebsiella InfectionsKlebsiella pneumoniaeMiceMicroscopy, FluorescenceMorpholinosOligonucleotides, AntisenseRadiopharmaceuticalsRNA, RibosomalTechnetiumTissue DistributionTomography, Emission-Computed, Single-PhotonTomography, X-Ray ComputedMorpholinosOligonucleotides, AntisenseRadiopharmaceuticalsRNA, RibosomalTechnetiumAntisense MORFBacterial infection imagingBacterial ribosomal RNA

Identifiers

PMID26832678
OpenAlexW2272897798

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.