ArticleScientific reports2016
Hierarchy of cellular decisions in collective behavior: Implications for wound healing.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- HB-EGF enhances collective cell migration via spatial coordination of traction.APL bioengineering · 2026Article
- Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration.Journal of functional biomaterials · 2025Article
- Junctional epithelium and hemidesmosomes: Tape and rivets for solving the "percutaneous device dilemma" in dental and other permanent implants.Bioactive materials · 2022Review
- Anti-Fibrotic Potential of Tomentosenol A, a Constituent of Cerumen from the Australian Native Stingless Bee,Antioxidants (Basel, Switzerland) · 2022Article
- Substrate stiffness engineered to replicate disease conditions influence senescence and fibrotic responses in primary lung fibroblasts.Frontiers in pharmacology · 2022Article
- Suppression of α-catenin and adherens junctions enhances epithelial cell proliferation and motility via TACE-mediated TGF-α autocrine/paracrine signaling.Molecular biology of the cell · 2021Article
- Matrix Stiffening Enhances DNCB-Induced IL-6 Secretion in Keratinocytes Through Activation of ERK and PI3K/Akt Pathway.Frontiers in immunology · 2021Article
- The keratin network of intermediate filaments regulates keratinocyte rigidity sensing and nuclear mechanotransduction.Science advances · 2021Article
- The Human Epidermal Basement Membrane: A Shaped and Cell Instructive Platform That Aging Slowly Alters.Biomolecules · 2020Review
- Article
- Scaffold stiffness influences breast cancer cell invasion via EGFR-linked Mena upregulation and matrix remodeling.Matrix biology : journal of the International Society for Matrix Biology · 2020Article
- Leader cell PLCγ1 activation during keratinocyte collective migration is induced by EGFR localization and clustering.Bioengineering & translational medicine · 2019Article
- Biomechanics of Collective Cell Migration in Cancer Progression: Experimental and Computational Methods.ACS biomaterials science & engineering · 2019Article
- Extended live-tracking and quantitative characterization of wound healing and cell migration with SiR-Hoechst.Experimental cell research · 2018Article
- Engineering Approaches to Study Cellular Decision Making.Annual review of biomedical engineering · 2018Review
- An investigation of the distribution and location of mast cells affected by the stiffness of substrates as a mechanical niche.International journal of biological sciences · 2018Article
- Immobilized epidermal growth factor stimulates persistent, directed keratinocyte migration via activation of PLCγ1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Collective processes such as wound re-epithelialization result from the integration of individual cellular decisions. To determine which individual cell behaviors represent the most promising targets to engineer re-epithelialization, we examined collective and individual responses of HaCaT keratinocytes seeded upon polyacrylamide gels of three stiffnesses (1, 30, and 100 kPa) and treated with a range of epidermal growth factor (EGF) doses. Wound closure was found to increase with substrate stiffness, but was responsive to EGF treatment only above a stiffness threshold. Individual cell behaviors were used to create a partial least squares regression model to predict the hierarchy of factors driving wound closure. Unexpectedly, cell area and persistence were found to have the strongest correlation to the observed differences in wound closure. Meanwhile, the model predicted a relatively weak correlation between wound closure with proliferation, and the unexpectedly minor input from proliferation was successfully tested with inhibition by aphidicolin. Combined, these results suggest that the poor clinical results for growth factor-based therapies for chronic wounds may result from a disconnect between the individual cellular behaviors targeted in these approaches and the resulting collective response. Additionally, the stiffness-dependency of EGF sensitivity suggests that therapies matched to microenvironmental characteristics will be more efficacious.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.