ReviewAmino acids2016
Amino acids in the cultivation of mammalian cells.
Review in Amino acids, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 117 citations in OpenAlex.
- Advances in Engineering Human Tissue Models.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Food-grade utilization of bovine slaughterhouse by-products for developing cost-effective media supplements in cultivated meat applications.Food science of animal resources · 2026Review
- Effects of culture media glucose concentration on LHCN-M2 skeletal muscle cell viability, differentiation and metabolic phenotype: a proof-of-concept study.Journal of muscle research and cell motility · 2026Article
- Untargeted metabolomics reveals metabolic reprogramming and key metabolites in adherent and suspension Vero cells.Frontiers in cell and developmental biology · 2026Article
- AutoML-Driven Soft Sensors for Real-Time Monitoring of Amino Acids in Mammalian Perfusion Cultures.Biotechnology and bioengineering · 2025Article
- Arginine: at the crossroads of nitrogen metabolism.The EMBO journal · 2025Review
- Novel Tripeptides as Tyrosinase Inhibitors: In Silico and In Vitro Approaches.International journal of molecular sciences · 2024Article
- Serum-Free Medium Supplemented withFoods (Basel, Switzerland) · 2024Article
- Effects of Melatonin, GM-CSF, IGF-1, and LIF in Culture Media on Embryonic Development: Potential Benefits of Individualization.International journal of molecular sciences · 2024Review
- Metabolic switching, growth kinetics and cell yields in the scalable manufacture of stem cell-derived insulin-producing cells.Stem cell research & therapy · 2024Article
- Mammalian cells internalize bacteriophages and use them as a resource to enhance cellular growth and survival.PLoS biology · 2023Article
- Scalable manufacturing of gene-modified human mesenchymal stromal cells with microcarriers in spinner flasks.Applied microbiology and biotechnology · 2023Article
- Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for sustainable cultured meat production.Scientific reports · 2023Article
- Split-Ring Resonator Based Sensor for the Detection of Amino Acids in Liquids.Sensors (Basel, Switzerland) · 2023Article
- Spent media analysis suggests cultivated meat media will require species and cell type optimization.NPJ science of food · 2022Article
- Aristolochic acid I induces proximal tubule injury through ROS/HMGB1/mt DNA mediated activation of TLRs.Journal of cellular and molecular medicine · 2022Article
- New perspectives in regenerative medicine and surgery: the bioactive composite therapies (BACTs).European journal of plastic surgery · 2022Article
- Structural Diversity of Di-Metalized Arginine Evidenced by Infrared Multiple Photon Dissociation (IRMPD) Spectroscopy in the Gas Phase.Molecules (Basel, Switzerland) · 2021Article
- Lactoyl leucine and isoleucine are bioavailable alternatives for canonical amino acids in cell culture media.Biotechnology and bioengineering · 2021Article
- Media on-demand: Continuous reconstitution of a chemically defined media directly from solids.Biotechnology and bioengineering · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amino acids are crucial for the cultivation of mammalian cells. This importance of amino acids was realized soon after the development of the first cell lines, and a solution of a mixture of amino acids has been supplied to cultured cells ever since. The importance of amino acids is further pronounced in chemically defined mammalian cell culture media, making the consideration of their biological and chemical properties necessary. Amino acids concentrations have been traditionally adjusted to their cellular consumption rates. However, since changes in the metabolic equilibrium of amino acids can be caused by changes in extracellular concentrations, metabolomics in conjunction with flux balance analysis is being used in the development of culture media. The study of amino acid transporters is also gaining importance since they control the intracellular concentrations of these molecules and are influenced by conditions in cell culture media. A better understanding of the solubility, stability, dissolution kinetics, and interactions of these molecules is needed for an exploitation of these properties in the development of dry powdered chemically defined media for mammalian cells. Due to the complexity of these mixtures however, this has proven to be challenging. Studying amino acids in mammalian cell culture media will help provide a better understanding of how mammalian cells in culture interact with their environment. It would also provide insight into the chemical behavior of these molecules in solutions of complex mixtures, which is important in the understanding of the contribution of individual amino acids to protein structure.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.