Evidence map›Paper›PMID 26832118›Full record

ArticleJournal of cardiovascular translational research2016

Nucleotide Catabolism on the Surface of Aortic Valve Xenografts; Effects of Different Decellularization Strategies.

Barbara Kutryb-Zajac, Ada H Y Yuen, Zain Khalpey, Paulina Zukowska, Ewa M Slominska, Patricia M Taylor, Steven Goldstein, Albert E Heacox, Marialuisa Lavitrano, Adrian H Chester and 2 more

Open access · hybridAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cardiovascular translational research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 4 countries.

Barbara Kutryb-ZajacDepartment of Biochemistry, Medical University of Gdansk, Gdańsk, Poland.
Ada H Y YuenHeart Science Centre, Imperial College London, London, UK.
Zain KhalpeyUniversity of Arizona, Tucson, AZ, USA.
Paulina ZukowskaDepartment of Biochemistry, Medical University of Gdansk, Gdańsk, Poland.
Ewa M SlominskaDepartment of Biochemistry, Medical University of Gdansk, Gdańsk, Poland.
Patricia M TaylorHeart Science Centre, Imperial College London, London, UK.
Steven GoldsteinCryoLife, Inc, Kennesaw, GA, USA.
Albert E HeacoxCryoLife, Inc, Kennesaw, GA, USA.
Marialuisa LavitranoUniversity Milano-Bicocca, Milan, Italy.
Adrian H ChesterHeart Science Centre, Imperial College London, London, UK.
Magdi H YacoubHeart Science Centre, Imperial College London, London, UK.
Ryszard T SmolenskiDepartment of Biochemistry, Medical University of Gdansk, Gdańsk, Poland. rt.smolenski@gmail.com.
Gdańsk Medical University · PLImperial College London · GBCryoLife (United States) · USUniversity of Arizona · USUniversity of Milano-Bicocca · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular nucleotide metabolism controls thrombosis and inflammation and may affect degeneration and calcification of aortic valve prostheses. We evaluated the effect of different decellularization strategies on enzyme activities involved in extracellular nucleotide metabolism. Porcine valves were tested intact or decellularized either by detergent treatment or hypotonic lysis and nuclease digestion. The rates of ATP hydrolysis, AMP hydrolysis, and adenosine deamination were estimated by incubation of aorta or valve leaflet sections with substrates followed by HPLC analysis. We demonstrated relatively high activities of ecto-enzymes on porcine valve as compared to the aortic wall. Hypotonic lysis/nuclease digestion preserved >80 % of ATP and AMP hydrolytic activity but reduced adenosine deamination to <10 %. Detergent decellularization completely removed (<5 %) all these activities. These results demonstrate high intensity of extracellular nucleotide metabolism on valve surface and indicate that various valve decellularization techniques differently affect ecto-enzyme activities that could be important in the development of improved valve prostheses.

Indexed as

BioprosthesisHeart Valve ProsthesisAdenosineAdenosine MonophosphateAdenosine TriphosphateAnimalsAortaAortic ValveChromatography, High Pressure LiquidDeaminationDeoxyribonuclease IDetergentsHeterograftsHydrolysisHypotonic SolutionsKineticsAdenosineAdenosine MonophosphateAdenosine TriphosphateDeoxyribonuclease IDetergentsHypotonic SolutionsNucleotidasesRibonuclease, PancreaticSodium Dodecyl SulfateAdenosineATP(Max 10) heart valvesNucleotide metabolismValve prosthesesXenografts

Identifiers

PMID26832118
PMCPMC4830859
OpenAlexW2254417895

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.