Evidence map›Paper›PMID 26831868›Full record

ArticleAuto- immunity highlights2016

Simultaneous detection of celiac disease-specific IgA antibodies and total IgA.

Kai Grossmann, Nadja Röber, Rico Hiemann, Stefan Rödiger, Peter Schierack, Dirk Reinhold, Martin W Laass, Karsten Conrad, Dirk Roggenbuck

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
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Article in Auto- immunity highlights, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Kai GrossmannGA Generic Assays GmbH, Dahlewitz, Germany.
Nadja RöberInstitute of Immunology, Medical Faculty, Technical University Dresden, Dresden, Germany.
Rico HiemannFaculty of Natural Sciences, Brandenburg University of Technology Cottbus-Senftenberg, Großenhainer Str. 57, 01968, Senftenberg, Germany.
Stefan RödigerFaculty of Natural Sciences, Brandenburg University of Technology Cottbus-Senftenberg, Großenhainer Str. 57, 01968, Senftenberg, Germany.
Peter SchierackFaculty of Natural Sciences, Brandenburg University of Technology Cottbus-Senftenberg, Großenhainer Str. 57, 01968, Senftenberg, Germany.
Dirk ReinholdInstitute of Molecular and Clinical Immunology, Otto-von-Guericke-University, Magdeburg, Germany.
Martin W LaassChildren's Hospital, Medical Faculty Carl Gustav Carus, Technical University Dresden, Dresden, Germany.
Karsten ConradInstitute of Immunology, Medical Faculty, Technical University Dresden, Dresden, Germany.
Dirk RoggenbuckGA Generic Assays GmbH, Dahlewitz, Germany. dirk.roggenbuck@b-tu.de.ORCID http://orcid.org/0000-0002-6861-6827
Brandenburg University of Technology Cottbus-Senftenberg · DEGeneric Assays (Germany) · DETechnische Universität Dresden · DEOtto-von-Guericke-Universität Magdeburg · DEUniversity Hospital Carl Gustav Carus · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeCeliac disease (CD) serology requires analysis of tissue transglutaminase type-2 (TG2autoAbs), deamidated gliadin (DGAbs), and as reference endomysial autoantibodies (EmA). Total IgA assessment helps to determine IgA-deficient CD patients. The novel multiplex indirect immunofluorescence (IIF) technique CytoBead was used to develop the first quantitative one-step serological CD assay comprising both simultaneous IgA autoAb and total IgA testing.

methodsCytoBead CeliAK detecting TG2autoAb, DGAb, EmA, and simultaneously total IgA uses fluorescent microparticles for antigen and antibody immobilization along with monkey-esophagus tissue sections on glass slides. The assay was interpreted visually by classical fluorescent microscopy and digital IIF using AKLIDES(®). Overall, 380 samples (155 CD patients, 5 with IgA deficiency, 68 with cystic fibrosis, 59 with eye disease, 93 blood donors) were run for performance analysis. Data were compared with classical IgA autoAb analysis by ELISA and IIF.

resultsComparing CD-specific IgA autoAb testing by CytoBead with classical IIF and ELISA, very good agreements for EmA, TG2autoAb, and DGAb were determined (Cohen's κ = 0.98, 0.96, 0.85, respectively). The difference between multiplex and single testing revealed a significant difference for TG2autoAb testing only (McNemar, p = 0.0078). Four CD patients and 4 controls demonstrated TG2autoAb positivity by ELISA but were negative by CytoBead. Further, 140/155 (90.9 %) CD patients demonstrated TG2autoAb levels above ten times the upper normal and all five IgA-deficient samples IgA levels <0.2 g/L by CytoBead.

conclusionsThe novel multiplex CytoBead CeliAK enables simultaneous CD-specific autoAb and IgA deficiency analyses comparable with classical testing by single-parameter assays. Thus, comprehensive CD serology by CytoBead can alleviate the workload in routine laboratories.

Indexed as

Celiac diseaseDeamidated gliadinDigital fluorescenceEndomysial antibodyTissue transglutaminase

Identifiers

PMID26831868
PMCPMC4733812
OpenAlexW2292121764

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.