Evidence map›Paper›PMID 26831405›Full record

ArticleThe Journal of international medical research2016

Protein microarray-mediated detection of antienterovirus antibodies in serum.

Aiying Zhang, Bingshui Xiu, Heqiu Zhang, Ning Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of international medical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Immunoproteomics: The Evolution from Classical Methods to Emerging Techniques.Methods in molecular biology (Clifton, N.J.) · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Aiying ZhangBeijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing, China.
Bingshui XiuDepartment of Vaccine Engineering, Institute of Basic Medical Sciences, Beijing, China.
Heqiu ZhangDepartment of Vaccine Engineering, Institute of Basic Medical Sciences, Beijing, China.
Ning LiBeijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing, China liningya@ccmu.edu.cn.
Institute of Basic Medical Sciences of the Chinese Academy of Medical SciencesBeijing YouAn Hospital · CNCapital Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo utilize prokaryotic gene expression and protein microarray to develop and evaluate a sensitive, accurate protein microarray assay for detecting antienterovirus antibodies in serum samples from patients with hand, foot and mouth disease (HFMD). Enterovirus 71 (EV71) and coxsackievirus A16 (CA16), two common causative agents for HFMD, were used for assay development.

methodsSerum was collected from patients with HFMD and healthy controls. EV71 and CA16 VP1 and VP3 genes were expressed in transfected Escherichia coli; the resultant VP1 and 3 proteins were used in a microarray assay for human serum EV71 and CA16 immunoglobulin (Ig) M and IgG. To validate the microarray assay, serum samples were tested for EV71 IgM using enzyme-linked immunosorbent assay (ELISA).

resultsOut of 50 patients with HFMD, EV71 IgM and CA16 IgM was detected in 80% and 44% of serum samples, respectively, using protein microarray, and EV71 IgM was detected in 78% of samples using ELISA. Protein microarray and ELISA showed 100% specificity for EV71-IgM detection.

conclusionThe protein microarray assay developed in the present study shows potential as a sensitive technique for detecting EV71 IgM in serum samples from patients with HFMD.

Indexed as

Antibodies, ViralCapsid ProteinsChild, PreschoolCloning, MolecularEnterovirusEnterovirus A, HumanEscherichia coliFemaleGene ExpressionHand, Foot and Mouth DiseaseHumansImmune SeraImmunoglobulin GImmunoglobulin MInfantMaleAntibodies, ViralCapsid ProteinsImmune SeraImmunoglobulin GImmunoglobulin MRecombinant ProteinsCA16EV71foot and mouth diseaseHandprotein microarray

Identifiers

PMID26831405
PMCPMC5580062
OpenAlexW2257231209

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.