ArticleProceedings of the National Academy of Sciences of the United States of America2016
Streamlined discovery of cross-linked chromatin complexes and associated histone modifications by mass spectrometry.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed.
- SAGA/ATAC complexes sustain aberrant chromatin regulation and promote tumorigenesis in diffuse midline glioma.bioRxiv : the preprint server for biology · 2026Article
- An E2 ubiquitin-conjugating enzyme links diubiquitinated H2B to H3K27M oncohistone function.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Variant Polycomb complexes inScience advances · 2022Article
- Review
- Examining histone modification crosstalk using immobilized libraries established from ligation-ready nucleosomes.Chemical science · 2020Article
- Native Chromatin Proteomics Reveals a Role for Specific Nucleoporins in Heterochromatin Organization and Maintenance.Molecular cell · 2020Article
- Levels of DNA cytosine methylation in thePeerJ · 2018Article
- Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes inGenes & development · 2017Article
- Unravelling the biology of chromatin in health and cancer using proteomic approaches.Expert review of proteomics · 2017Review
- Article
- Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins.Molecular cell · 2016Article
- Proteomics to study DNA-bound and chromatin-associated gene regulatory complexes.Human molecular genetics · 2016Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Posttranslational modifications (PTMs) are key contributors to chromatin function. The ability to comprehensively link specific histone PTMs with specific chromatin factors would be an important advance in understanding the functions and genomic targeting mechanisms of those factors. We recently introduced a cross-linked affinity technique, BioTAP-XL, to identify chromatin-bound protein interactions that can be difficult to capture with native affinity techniques. However, BioTAP-XL was not strictly compatible with similarly comprehensive analyses of associated histone PTMs. Here we advance BioTAP-XL by demonstrating the ability to quantify histone PTMs linked to specific chromatin factors in parallel with the ability to identify nonhistone binding partners. Furthermore we demonstrate that the initially published quantity of starting material can be scaled down orders of magnitude without loss in proteomic sensitivity. We also integrate hydrophilic interaction chromatography to mitigate detergent carryover and improve liquid chromatography-mass spectrometric performance. In summary, we greatly extend the practicality of BioTAP-XL to enable comprehensive identification of protein complexes and their local chromatin environment.
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Registered trials
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