Evidence map›Paper›PMID 26831041›Full record

ArticleTissue engineering. Part C, Methods2016

A Self-Folding Hydrogel In Vitro Model for Ductal Carcinoma.

Hye Rin Kwag, Janna V Serbo, Preethi Korangath, Saraswati Sukumar, Lewis H Romer, David H Gracias

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering. Part C, Methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hye Rin Kwag1 Department of Chemical and Biomolecular Engineering, Johns Hopkins University , Baltimore, Maryland.
Janna V Serbo2 Department of Biomedical Engineering, Johns Hopkins University School of Medicine , Baltimore, Maryland.
Preethi Korangath3 Department of Oncology, Johns Hopkins University School of Medicine , Baltimore, Maryland.
Saraswati Sukumar3 Department of Oncology, Johns Hopkins University School of Medicine , Baltimore, Maryland.
Lewis H Romer2 Department of Biomedical Engineering, Johns Hopkins University School of Medicine , Baltimore, Maryland.
David H Gracias1 Department of Chemical and Biomolecular Engineering, Johns Hopkins University , Baltimore, Maryland.

Funding

Raman spectroscopy probes for assisting stereotactic breast biopsy needle placemeR43CA141868 · NCI · NEWTON LABORATORIES · PI FULGHUM, STEPHEN FREDERICK · 2009 to 2009
$100k
Anisotropy in Matrix Bioblocks: A Platform for Microvascular EngineeringF31EB018187 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI SERBO, JANNA VICTOROVNA · 2014 to 2015
$67k
NCI NIH HHS U54CA141868NIBIB NIH HHS F31 EB018187
6 · The paper itself

Abstract

A significant challenge in oncology is the need to develop in vitro models that accurately mimic the complex microenvironment within and around normal and diseased tissues. Here, we describe a self-folding approach to create curved hydrogel microstructures that more accurately mimic the geometry of ducts and acini within the mammary glands, as compared to existing three-dimensional block-like models or flat dishes. The microstructures are composed of photopatterned bilayers of poly (ethylene glycol) diacrylate (PEGDA), a hydrogel widely used in tissue engineering. The PEGDA bilayers of dissimilar molecular weights spontaneously curve when released from the underlying substrate due to differential swelling ratios. The photopatterns can be altered via AutoCAD-designed photomasks so that a variety of ductal and acinar mimetic structures can be mass-produced. In addition, by co-polymerizing methacrylated gelatin (methagel) with PEGDA, microstructures with increased cell adherence are synthesized. Biocompatibility and versatility of our approach is highlighted by culturing either SUM159 cells, which were seeded postfabrication, or MDA-MB-231 cells, which were encapsulated in hydrogels; cell viability is verified over 9 and 15 days, respectively. We believe that self-folding processes and associated tubular, curved, and folded constructs like the ones demonstrated here can facilitate the design of more accurate in vitro models for investigating ductal carcinoma.

Indexed as

Models, BiologicalCarcinoma, DuctalCell AdhesionCell Line, TumorCells, ImmobilizedHumansHydrogelsPolyethylene GlycolsHydrogelspolyethyleneglycol 400 diacrylatePolyethylene Glycols

Identifiers

PMID26831041
PMCPMC4827285

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.