ArticleJournal of lipid research2016
Novel flow cytometric approach for the detection of adipocyte subpopulations during adipogenesis.
Article in Journal of lipid research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
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- Differential lipid metabolism in beef cattle: A comparative study of high and low residual feed intake bulls.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- CRISPR-Cas9 screening reveals microproteins regulating adipocyte proliferation and lipid metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- BCR::ABL1 expression in chronic myeloid leukemia cells in low oxygen is regulated by glutamine via CD36-mediated fatty acid uptake.Cancer cell international · 2025Article
- A simple, reliable and easily generalizable cell-based assay for screening potential drugs that inhibit lipid accumulation.Current research in toxicology · 2025Article
- Isolation and Characterization of Equine Mesenchymal Stromal/Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Quantification of Adipogenesis Using Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status.Journal of functional biomaterials · 2021Article
- Tanshinone IIA Downregulates Lipogenic Gene Expression and Attenuates Lipid Accumulation through the Modulation of LXRα/SREBP1 Pathway in HepG2 Cells.Biomedicines · 2021Article
- Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells.Cells · 2021Article
- The Role of Pref-1 during Adipogenic Differentiation: An Overview of Suggested Mechanisms.International journal of molecular sciences · 2020Review
- Adipocyte-Based Cell Therapy in Oncology: The Role of Cancer-Associated Adipocytes and Their Reinterpretation as Delivery Platforms.Pharmaceutics · 2020Review
- Single-cell RNA-seq highlights heterogeneity in human primary Wharton's jelly mesenchymal stem/stromal cells cultured in vitro.Stem cell research & therapy · 2020Article
- Evolution of ASC Immunophenotypical Subsets During Expansion In Vitro.International journal of molecular sciences · 2020Article
- The Effect of Early Rounds of ex vivo Expansion and Cryopreservation on the Adipogenic Differentiation Capacity of Adipose-Derived Stromal/Stem Cells.Scientific reports · 2019Article
- Human bone marrow adipocytes display distinct immune regulatory properties.EBioMedicine · 2019Article
- Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells.Biomimetics (Basel, Switzerland) · 2019Article
- Reference Gene Expression in Adipose-Derived Stromal Cells Undergoing Adipogenic Differentiation.Tissue engineering. Part C, Methods · 2019Article
- Human Adipose-Derived Hydrogel Characterization Based onStem cells international · 2019Article
- Identification of transcription factors potentially involved in human adipogenesis in vitro.Molecular genetics & genomic medicine · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of mesenchymal stromal cells (MSCs) to differentiate into adipocytes provides a cellular model of human origin to study adipogenesis in vitro. One of the major challenges in studying adipogenesis is the lack of tools to identify and monitor the differentiation of various subpopulations within the heterogeneous pool of MSCs. Cluster of differentiation (CD)36 plays an important role in the formation of intracellular lipid droplets, a key characteristic of adipocyte differentiation/maturation. The objective of this study was to develop a reproducible quantitative method to study adipocyte differentiation by comparing two lipophilic dyes [Nile Red (NR) and Bodipy 493/503] in combination with CD36 surface marker staining. We identified a subpopulation of adipose-derived stromal cells that express CD36 at intermediate/high levels and show that combining CD36 cell surface staining with neutral lipid-specific staining allows us to monitor differentiation of adipose-derived stromal cells that express CD36(intermediate/high)during adipocyte differentiation in vitro. The gradual increase of CD36(intermediate/high/)NR(positive)cells during the 21 day adipogenesis induction period correlated with upregulation of adipogenesis-associated gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.