ArticleScientific reports2016
Quantitative phospho-proteomics reveals the Plasmodium merozoite triggers pre-invasion host kinase modification of the red cell cytoskeleton.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 49 citations in OpenAlex.
- Detection of malaria infection from parasite-free blood smears.Malaria journal · 2026Article
- In silico analysis of the invasion mechanics and invasiveness of the Plasmodium falciparum merozoite.Biomechanics and modeling in mechanobiology · 2026Article
- Article
- Basigin mediation of Plasmodium falciparum red blood cell invasion does not require its transmembrane domain or interaction with monocarboxylate transporter 1.PLoS pathogens · 2024Article
- Pharmacological activation of PIEZO1 in human red blood cells prevents Plasmodium falciparum invasion.Cellular and molecular life sciences : CMLS · 2023Article
- Proteomic Analysis of the Role of the Adenylyl Cyclase-cAMP Pathway in Red Blood Cell Mechanical Responses.Cells · 2022Article
- RBC membrane biomechanics and Plasmodium falciparum invasion: probing beyond ligand-receptor interactions.Trends in parasitology · 2022Review
- Generation of red blood cells from stem cells: Achievements, opportunities and perspectives for malaria research.Frontiers in cellular and infection microbiology · 2022Review
- Proteome of Stored RBC Membrane and Vesicles from Heterozygous Beta Thalassemia Donors.International journal of molecular sciences · 2021Article
- 20S proteasomes secreted by the malaria parasite promote its growth.Nature communications · 2021Article
- Simvastatin protects heart function and myocardial energy metabolism in pulmonary arterial hypertension induced right heart failure.Journal of bioenergetics and biomembranes · 2021Article
- Pathogen induced subversion of NADCell death discovery · 2021Article
- Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion.Scientific reports · 2020Article
- An exported kinase family mediates species-specific erythrocyte remodelling and virulence in human malaria.Nature microbiology · 2020Article
- The effects of dyslipidaemia and cholesterol modulation on erythrocyte susceptibility to malaria parasite infection.Malaria journal · 2019Article
- Host Cytoskeleton Remodeling throughout the Blood Stages of Plasmodium falciparum.Microbiology and molecular biology reviews : MMBR · 2019Review
- Plasmodium myosin A drives parasite invasion by an atypical force generating mechanism.Nature communications · 2019Article
- Red Blood Cells: Chasing Interactions.Frontiers in physiology · 2019Review
- Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles usingInternational journal of nanomedicine · 2019Article
- Piezo1 mediates angiogenesis through activation of MT1-MMP signaling.American journal of physiology. Cell physiology · 2019Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The invasive blood-stage malaria parasite - the merozoite - induces rapid morphological changes to the target erythrocyte during entry. However, evidence for active molecular changes in the host cell that accompany merozoite invasion is lacking. Here, we use invasion inhibition assays, erythrocyte resealing and high-definition imaging to explore red cell responses during invasion. We show that although merozoite entry does not involve erythrocyte actin reorganisation, it does require ATP to complete the process. Towards dissecting the ATP requirement, we present an in depth quantitative phospho-proteomic analysis of the erythrocyte during each stage of invasion. Specifically, we demonstrate extensive increased phosphorylation of erythrocyte proteins on merozoite attachment, including modification of the cytoskeletal proteins beta-spectrin and PIEZO1. The association with merozoite contact but not active entry demonstrates that parasite-dependent phosphorylation is mediated by host-cell kinase activity. This provides the first evidence that the erythrocyte is stimulated to respond to early invasion events through molecular changes in its membrane architecture.
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