Evidence map›Paper›PMID 26830451›Full record

ArticleAging2016

DNA damage markers in dermal fibroblasts in vitro reflect chronological donor age.

Mariëtte E C Waaijer, Eleonora Croco, Rudi G J Westendorp, P Eline Slagboom, John M Sedivy, Antonello Lorenzini, Andrea B Maier

Open access · hybridAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Aging, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

  1. Trial
  2. Review
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  4. Review
  5. Article
  6. Review
  7. Article
  8. Obesity May Accelerate the Aging Process.Frontiers in endocrinology · 2019
    Review
  9. Article
  10. Review
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 6 institutions in 5 countries.

Mariëtte E C WaaijerDepartment of Gerontology and Geriatrics, Leiden University Medical Center, 2300 RC Leiden, the Netherlands.
Eleonora CrocoDepartment for Life Quality Studies, University of Bologna, 40126 Bologna, Italy.
Rudi G J WestendorpDepartment of Public Health and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, 1123 Copenhagen, Denmark.
P Eline SlagboomDepartment of Molecular Epidemiology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
John M SedivyDepartment of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02903, USA.
Antonello LorenziniDepartment of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, Italy.
Andrea B MaierDepartment of Internal Medicine, Section of Gerontology and Geriatrics, VU University Medical Center, 1007 MB Amsterdam, The Netherlands.
University of Bologna · ITBrown University · USLeiden University Medical Center · NLNetherlands Consortium for Healthy Ageing · NLThe Royal Melbourne Hospital · AUUniversity of Copenhagen · DK

Funding

Effectors of cellular senescence statesR37AG016694 · NIA · BROWN UNIVERSITY · PI SEDIVY, JOHN M · 2009 to 2018
$3.2M
NIA NIH HHS R37 AG016694
6 · The paper itself

Abstract

The aging process is accompanied by an accumulation of cellular damage, which compromises the viability and function of cells and tissues. We aim to further explore the association between in vitro DNA damage markers and the chronological age of the donor, as well as long-lived family membership and presence of cardiovascular diseases. Therefore, numbers of 53BP1 foci, telomere-associated foci (TAF) and micronuclei were measured in cultured dermal fibroblasts obtained from three age groups of donors (mean age 22, 63 and 90 years). Fibroblasts were cultured without a stressor and with 0.6 μM rotenone for 3 days. We found that 53BP1 foci and TAF were more frequently present in fibroblasts of old donors compared to middle-aged and young donors. No association between micronuclei and donor age was found. Within the fibroblasts of the middle-aged donors we did not find associations between DNA damage markers and long-lived family membership or cardiovascular disease. Results were comparable when fibroblasts were stressed in vitro with rotenone. In conclusion, we found that DNA damage foci of cultured fibroblasts are significantly associated with the chronological age, but not biological age, of the donor.

Indexed as

Cellular SenescenceDNA DamageAgedAged, 80 and overAge FactorsAgingCells, CulturedFemaleFibroblastsHumansMaleMicronuclei, Chromosome-DefectiveMiddle AgedRotenoneTelomereTime FactorsRotenoneTP53BP1 protein, humanTumor Suppressor p53-Binding Protein 153BP1biological agehuman agingmicronucleitelomere-associated foci

Identifiers

PMID26830451
PMCPMC4761719
OpenAlexW2418995811

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.