ArticleScientific reports2016
Loop replacements with gut-binding peptides in Cry1Ab domain II enhanced toxicity against the brown planthopper, Nilaparvata lugens (Stål).
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Three Decades of China's Bt Cotton: Achievements and Insights.Plant biotechnology journal · 2026Review
- Generation of an Insecticidal Human Domain Antibody from a Phage Library TargetingCurrent issues in molecular biology · 2026Article
- Synergistic Insecticidal Activity AgainstInternational journal of molecular sciences · 2025Article
- Domain IIIToxins · 2024Article
- The crystal structure of Cry78Aa from Bacillus thuringiensis provides insights into its insecticidal activity.Communications biology · 2022Article
- Stability is essential for insecticidal activity of Vip3Aa toxin against Spodoptera exigua.AMB Express · 2022Article
- Peptide mediated, enhanced toxicity of a bacterial pesticidal protein against southern green stink bug.Microbial biotechnology · 2022Article
- Recombinant Mosquito Densovirus withToxins · 2022Article
- Engineering of Cry3Bb1 provides mechanistic insights toward countering western corn rootworm resistance.Current research in insect science · 2022Article
- Role of Lectin in the Response ofFrontiers in immunology · 2022Review
- Genetic Modification Approaches for ParasporinsMolecules (Basel, Switzerland) · 2021Review
- Evolution-aided engineering of plant specialized metabolism.aBIOTECH · 2021Review
- Which Is Stronger? A Continuing Battle Between Cry Toxins and Insects.Frontiers in microbiology · 2021Review
- Streamlined phage display library protocols for identification of insect gut binding peptides highlight peptide specificity.Current research in insect science · 2021Article
- Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action.Toxins · 2020Review
- Article
- Crystal structure of Bacillus thuringiensis Cry7Ca1 toxin active against Locusta migratoria manilensis.Protein science : a publication of the Protein Society · 2019Article
- Applications of Protein Engineering and Directed Evolution in Plant Research.Plant physiology · 2019Review
- Expression of hybrid fusion protein (Cry1Ac::ASAL) in transgenic rice plants imparts resistance against multiple insect pests.Scientific reports · 2018Article
- Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity.Scientific reports · 2018Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus thuringiensis (Bt) Cry toxins have been used widely in pest managements. However, Cry toxins are not effective against sap-sucking insects (Hemiptera), which limits the application of Bt for pest management. In order to extend the insecticidal spectrum of Bt toxins to the rice brown planthopper (BPH), Nilaparvata lugens, we modified Cry1Ab putative receptor binding domains with selected BPH gut-binding peptides (GBPs). Three surface exposed loops in the domain II of Cry1Ab were replaced with two GBPs (P2S and P1Z) respectively. Bioassay results showed that toxicity of modified toxin L2-P2S increased significantly (~9 folds) against BPH nymphs. In addition, damage of midgut cells was observed from the nymphs fed with L2-P2S. Our results indicate that modifying Cry toxins based on the toxin-gut interactions can broaden the insecticidal spectrum of Bt toxin. This method provides another approach for the development of transgenic crops with novel insecticidal activity against hemipteran insects and insect populations resistant to current Bt transgenic crops.
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