ArticleEpigenetics2015
Identification of a new locus and validation of previously reported loci showing differential methylation associated with smoking. The REGICOR study.
Article in Epigenetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it, 46 citations in OpenAlex.
- Physical Activity and Genome-wide DNA Methylation: The REgistre GIroní del COR Study.Medicine and science in sports and exercise · 2020Pooled it
- DNA methylation signatures of illicit drug injection and hepatitis C are associated with HIV frailty.Nature communications · 2017Pooled it
- Hypoxia shapes both therapeutic response and resistance in metastatic clear cell renal cell carcinoma.Cancer cell · 2026Article
- Cohort Profile: The Girona Heart Registry.International journal of epidemiology · 2025Article
- Waterpipe and cigarette epigenome analysis reveals markers implicated in addiction and smoking type inference.Environment international · 2023Article
- Transcriptomic responses in the blood and sputum of cigarette smokers compared to e-cigarette vapers.Respiratory research · 2023Article
- DNA-Methylation Signatures of Tobacco Smoking in a High Cardiovascular Risk Population: Modulation by the Mediterranean Diet.International journal of environmental research and public health · 2023Article
- Machine Learning Approximations to Predict Epigenetic Age Acceleration in Stroke Patients.International journal of molecular sciences · 2023Article
- Dissecting the epigenomic differences between smoking and nicotine dependence in a veteran cohort.Addiction biology · 2023Article
- Epigenetics and Gut Microbiota Crosstalk: A potential Factor in Pathogenesis of Cardiovascular Disorders.Bioengineering (Basel, Switzerland) · 2022Review
- Sex-specific DNA methylation: impact on human health and development.Molecular genetics and genomics : MGG · 2022Review
- Sex-specific associations with DNA methylation in lung tissue demonstrate smoking interactions.Epigenetics · 2021Article
- DNA methylation biomarkers of myocardial infarction and cardiovascular disease.Clinical epigenetics · 2021Article
- Epigenome-wide change and variation in DNA methylation in childhood: trajectories from birth to late adolescence.Human molecular genetics · 2021Article
- Cigarette smoke-induced alterations in blood: A review of research on DNA methylation and gene expression.Experimental and clinical psychopharmacology · 2021Review
- Genetic Control of Human Infection with SARS-CoV-2.Russian journal of genetics · 2021Article
- Current status of development of methylation biomarkers for in vitro diagnostic IVD applications.Clinical epigenetics · 2020Review
- Epigenome-wide association study of depression symptomatology in elderly monozygotic twins.Translational psychiatry · 2019Article
- A combined epigenome- and transcriptome-wide association study of the oral masticatory mucosa assigns CYP1B1 a central role for epithelial health in smokers.Clinical epigenetics · 2019Article
- Epigenome-wide association study of serum cotinine in current smokers reveals novel genetically driven loci.Clinical epigenetics · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 9 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Smoking increases the risk of many diseases and could act through changes in DNA methylation patterns. The aims of this study were to determine the association between smoking and DNA methylation throughout the genome at cytosine-phosphate-guanine (CpG) site level and genomic regions. A discovery cross-sectional epigenome-wide association study nested in the follow-up of the REGICOR cohort was designed and included 645 individuals. Blood DNA methylation was assessed using the Illumina HumanMethylation450 BeadChip. Smoking status was self-reported using a standardized questionnaire. We identified 66 differentially methylated CpG sites associated with smoking, located in 38 genes. In most of these CpG sites, we observed a trend among those quitting smoking to recover methylation levels typical of never smokers. A CpG site located in a novel smoking-associated gene (cg06394460 in LNX2) was hypomethylated in current smokers. Moreover, we validated two previously reported CpG sites (cg05886626 in THBS1, and cg24838345 in MTSS1) for their potential relation to atherosclerosis and cancer diseases, using several different approaches: CpG site methylation, gene expression, and plasma protein level determinations. Smoking was also associated with higher THBS1 gene expression but with lower levels of thrombospondin-1 in plasma. Finally, we identified differential methylation regions in 13 genes and in four non-coding RNAs. In summary, this study replicated previous findings and identified and validated a new CpG site located in LNX2 associated with smoking.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.