Evidence map›Paper›PMID 26828681›Full record

ArticleBiomaterials2016

Nanoparticle-mediated transcriptional modification enhances neuronal differentiation of human neural stem cells following transplantation in rat brain.

Xiaowei Li, Stephany Y Tzeng, Xiaoyan Liu, Markus Tammia, Yu-Hao Cheng, Andrew Rolfe, Dong Sun, Ning Zhang, Jordan J Green, Xuejun Wen and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xiaowei LiTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Stephany Y TzengTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Xiaoyan LiuDepartment of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA.
Markus TammiaTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Yu-Hao ChengTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Andrew RolfeDepartment of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA.
Dong SunDepartment of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA.
Ning ZhangDepartment of Biomedical Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA.
Jordan J GreenTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Xuejun WenDepartment of Chemical and Life Science Engineering, School of Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA. Electronic address: xwen@vcu.edu.
Hai-Quan MaoTranslational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; Department of Materials Science & Engineering, Johns Hopkins University, Baltimore, MD 21218, USA. Electronic address: hmao@jhu.edu.

Funding

Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI Jiang Qian · 1985 to 2026
$22.1M
Gene Delivery Nanoparticles to Treat GlioblastomaR01EB016721 · NIBIB · JOHNS HOPKINS UNIVERSITY · PI GREEN, JORDAN · 2013 to 2017
$1.9M
Directed In Vivo Differentiation of Neural Stem Cells for Repair of Brain LesionR21NS085714 · NINDS · JOHNS HOPKINS UNIVERSITY · PI MAO, HAI-QUAN · 2014 to 2015
$446k
Induced Pluripotent Stem Cells from Neurosurgical removed tissue for Brain RepairR21NS078710 · NINDS · VIRGINIA COMMONWEALTH UNIVERSITY · PI RAO, RAJ RAGHAVENDRA, SUN, DONG · 2012 to 2013
$399k
NEI NIH HHS P30 EY001765NIBIB NIH HHS 1R01EB016721NIBIB NIH HHS R01 EB016721NINDS NIH HHS R21 NS078710NINDS NIH HHS R21NS078710-02NINDS NIH HHS R21 NS085714NINDS NIH HHS R21NS085714
6 · The paper itself

Abstract

Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue-derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy.

Indexed as

Cell DifferentiationStem Cell TransplantationTranscription, GeneticAnimalsBrainCell SurvivalHumansNanoparticlesNeural Stem CellsNeuronsPolymersRats, NudeTransfectionpoly(beta-amino ester)PolymersNanoparticlesNeurogenin-2Neuronal differentiationTransfectionTraumatic brain injury

Identifiers

PMID26828681
PMCPMC4755890

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.