ArticleScientific reports2016
Toward Contactless Biology: Acoustophoretic DNA Transfection.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 72 citations in OpenAlex.
- Semidefinite programming for manipulating acoustic traps in real time (SMART).Scientific reports · 2025Article
- Sound innovations for biofabrication and tissue engineering.Microsystems & nanoengineering · 2024Review
- Coalescence and mixing dynamics of droplets in acoustic levitation by selective colour imaging and measurement.Scientific reports · 2023Article
- Microfluidic platform using focused ultrasound passing through hydrophobic meshes with jump availability.PNAS nexus · 2023Article
- Acoustic-propelled micro/nanomotors and nanoparticles for biomedical research, diagnosis, and therapeutic applications.Frontiers in bioengineering and biotechnology · 2023Review
- Airborne ultrasound pulse amplification based on acoustic resonance switching.Scientific reports · 2022Article
- Effective Cell Transfection in An Ultrasonically Levitated Droplet for Sustainable Technology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Oscillation Dynamics of Multiple Water Droplets Levitated in an Acoustic Field.Micromachines · 2022Article
- High-speed acoustic holography with arbitrary scattering objects.Science advances · 2022Article
- Dynamic Acoustic Levitator Based On Subwavelength Aperture Control.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Contactless acoustic micro/nano manipulation: a paradigm for next generation applications in life sciences.Proceedings. Mathematical, physical, and engineering sciences · 2020Review
- Acoustic Microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2020Review
- Article
- Coalescence Dynamics of Acoustically Levitated Droplets.Micromachines · 2020Article
- Evaporation and drying kinetics of water-NaCl dropletsRSC advances · 2020Article
- Acoustic Manipulation of Droplets under Reduced Gravity.Scientific reports · 2019Article
- Contactless, programmable acoustofluidic manipulation of objects on water.Lab on a chip · 2019Article
- Two-Dimensional Manipulation in Mid-Air Using a Single Transducer Acoustic Levitator.Micromachines · 2019Article
- Evaporation of droplet in mid-air: Pure and binary droplets in single-axis acoustic levitator.PloS one · 2019Article
- Acoustic tweezers for the life sciences.Nature methods · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acoustophoresis revolutionized the field of container-less manipulation of liquids and solids by enabling mixing procedures which avoid contamination and loss of reagents due to the contact with the support. While its applications to chemistry and engineering are straightforward, additional developments are needed to obtain reliable biological protocols in a contactless environment. Here, we provide a first, fundamental step towards biological reactions in air by demonstrating the acoustophoretic DNA transfection of mammalian cells. We developed an original acoustophoretic design capable of levitating, moving and mixing biological suspensions of living mammalians cells and of DNA plasmids. The precise and sequential delivery of the mixed solutions into tissue culture plates is actuated by a novel mechanism based on the controlled actuation of the acoustophoretic force. The viability of the contactless procedure is tested using a cellular model sensitive to small perturbation of neuronal differentiation pathways. Additionally, the efficiency of the transfection procedure is compared to standard, container-based methods for both single and double DNA transfection and for different cell types including adherent growing HeLa cancer cells, and low adhesion neuron-like PC12 cells. In all, this work provides a proof of principle which paves the way to the development of high-throughput acoustophoretic biological reactors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.