Evidence map›Paper›PMID 26828225›Full record

ArticleRNA biology2017

Cellular mRNA recruits the ribosome via eIF3-PABP bridge to initiate internal translation.

Nehal Thakor, M Duane Smith, Luc Roberts, Mame Daro Faye, Harshil Patel, Hans-Joachim Wieden, Jamie H D Cate, Martin Holcik

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 41 citations in OpenAlex.

  1. Article
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  10. Article
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  12. Article
  13. IRES Trans-Acting Factors, Key Actors of the Stress Response.International journal of molecular sciences · 2019
    Review
  14. Regulation of alternative splicing in obesity-induced hypertension.Diabetes, metabolic syndrome and obesity : targets and therapy · 2019
    Article
  15. Review
  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Nehal Thakora Apoptosis Research Center , Children's Hospital of Eastern Ontario Research Institute , Ottawa , Ontario , Canada.
M Duane Smithd Department of Molecular and Cell Biology , University of California , Berkeley , CA , USA.
Luc Robertsc Department of Chemistry and Biochemistry , Alberta RNA Research and Training Institute, University of Lethbridge , Lethbridge , AB , Canada.
Mame Daro Fayea Apoptosis Research Center , Children's Hospital of Eastern Ontario Research Institute , Ottawa , Ontario , Canada.
Harshil Patelc Department of Chemistry and Biochemistry , Alberta RNA Research and Training Institute, University of Lethbridge , Lethbridge , AB , Canada.
Hans-Joachim Wiedenc Department of Chemistry and Biochemistry , Alberta RNA Research and Training Institute, University of Lethbridge , Lethbridge , AB , Canada.
Jamie H D Cated Department of Molecular and Cell Biology , University of California , Berkeley , CA , USA.
Martin Holcika Apoptosis Research Center , Children's Hospital of Eastern Ontario Research Institute , Ottawa , Ontario , Canada.
Children's Hospital of Eastern Ontario · CAUniversity of Lethbridge · CAUniversity of California, Berkeley · US

Funding

CIHR MOP 89737
6 · The paper itself

Abstract

IRES-mediated translation of key cell fate regulating genes has been implicated in tumorigenesis. Concerted action of canonical eukaryotic initiation factors and IRES transacting factors (ITAFs) was shown to regulate cellular IRES mediated translation; however, the precise molecular mechanism of ribosome recruitment to cellular IRESes remains unclear. Here we show that the X-linked inhibitor of apoptosis (XIAP) IRES operates in an evolutionary conserved viral like mode and the structural integrity, particularly in the vicinity of AUG, is critical for ribosome recruitment. The binding of eIF3 together with PABP potentiates ribosome recruitment to the IRES. Our data support the model in which eIF3 binds directly to the XIAP IRES RNA in a structure-dependent manner and acts as a scaffold for IRES RNA, PABP and the 40S ribosome.

Indexed as

Internal Ribosome Entry SitesApoptosisCodon, InitiatorEukaryotic Initiation Factor-3Eukaryotic Initiation FactorsHeLa CellsHumansPoly(A)-Binding ProteinsProtein BiosynthesisRibosomesRibosome Subunits, Small, EukaryoticRNA, MessengerX-Linked Inhibitor of Apoptosis ProteinCodon, InitiatorEukaryotic Initiation Factor-3Eukaryotic Initiation FactorsInternal Ribosome Entry SitesPoly(A)-Binding ProteinsRNA, MessengerXIAP protein, humanX-Linked Inhibitor of Apoptosis ProteinCellular IRESeIF3NMIA-SHAPEPABPRNA structuretoeprinting assayXIAP

Identifiers

PMID26828225
PMCPMC5449081
OpenAlexW2330185538

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.