ReviewMethods (San Diego, Calif.)2016
Fixed and live visualization of RNAs in Drosophila oocytes and embryos.
Review in Methods (San Diego, Calif.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Dynamics of bicoid mRNA localisation and translation dictate morphogen gradient formation.The EMBO journal · 2026Article
- Discrete 3' UTR sequence motifs regulate nanos RNA enrichment in Drosophila germ granules.Developmental biology · 2026Article
- Lighting Up Endogenous RNA: Fluorescent Aptamer Sensors for Live-Cell Imaging.Chemical & biomedical imaging · 2026Review
- Spatial organization of translation and translational repression in two phases of germ granules.Nature communications · 2024Article
- Direct observation of translational activation by a ribonucleoprotein granule.Nature cell biology · 2024Article
- Catalytic activity of the Bin3/MePCE methyltransferase domain is dispensable for 7SK snRNP function in Drosophila melanogaster.Genetics · 2024Article
- Germ Granule Evolution Provides Mechanistic Insight into Drosophila Germline Development.Molecular biology and evolution · 2023Article
- Localization of oskar mRNA by agglomeration in ribonucleoprotein granules.PLoS genetics · 2023Article
- Plasticity of Drosophila germ granules during germ cell development.PLoS biology · 2023Article
- Computational modeling offers new insight into Drosophila germ granule development.Biophysical journal · 2022Article
- Knock down analysis reveals critical phases for specific oskar noncoding RNA functions during Drosophila oogenesis.G3 (Bethesda, Md.) · 2021Article
- Different roles for the adjoining and structurally similar A-rich and poly(A) domains of oskar mRNA: Only the A-rich domain is required for oskar noncoding RNA function, which includes MTOC positioning.Developmental biology · 2021Article
- Opposing roles for Egalitarian and Staufen in transport, anchoring and localization of oskar mRNA in the Drosophila oocyte.PLoS genetics · 2021Article
- The ELAV/Hu protein Found in neurons regulates cytoskeletal and ECM adhesion inputs for space-filling dendrite growth.PLoS genetics · 2020Article
- Illuminating RNA Biology: Tools for Imaging RNA in Live Mammalian Cells.Cell chemical biology · 2020Review
- CompartmentalizedeLife · 2020Article
- Uncoupling of nucleo-cytoplasmic RNA export and localization during stress.Nucleic acids research · 2019Article
- PINK1 Inhibits Local Protein Synthesis to Limit Transmission of Deleterious Mitochondrial DNA Mutations.Molecular cell · 2019Article
- A Gene Expression Screen inG3 (Bethesda, Md.) · 2019Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The ability to visualize RNA in situ is essential to dissect mechanisms for the temporal and spatial regulation of gene expression that drives development. Although considerable attention has been focused on transcriptional control, studies in model organisms like Drosophila have highlighted the importance of post-transcriptional mechanisms - most notably intracellular mRNA localization - in the formation and patterning of the body axes, specification of cell fates, and polarized cell functions. Our understanding of both types of regulation has been greatly advanced by technological innovations that enable a combination of highly quantitative and dynamic analysis of RNA. This review presents two methods, single molecule fluorescence in situ hybridization for high resolution quantitative RNA detection in fixed Drosophila oocytes and embryos and genetically encoded fluorescent RNA labeling for detection in live cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.