ArticleJournal of immunological methods2016
T-cell libraries allow simple parallel generation of multiple peptide-specific human T-cell clones.
Article in Journal of immunological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Oligoclonal B cell expansion and passenger fusion genes predict response to Nivolumab in recurrent ovarian cancer: phase II Kyoto trial.Cancer immunology, immunotherapy : CII · 2026Trial
- Optimized procurement of cancer-reactive T-cell receptors from clinical samples.Immunotherapy advances · 2026Article
- Review
- Structural definition of HLA class II-presented SARS-CoV-2 epitopes reveals a mechanism to escape pre-existing CD4Cell reports · 2023Article
- Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- CD4Cell reports · 2020Article
- Review
- The Ways of Isolating Neoantigen-Specific T Cells.Frontiers in oncology · 2020Review
- GPU-Accelerated Discovery of Pathogen-Derived Molecular Mimics of a T-Cell Insulin Epitope.Frontiers in immunology · 2020Article
- Human leukocyte antigen (HLA) class II peptide flanking residues tune the immunogenicity of a human tumor-derived epitope.The Journal of biological chemistry · 2019Article
- T-cell Receptors EngineeredCancer immunology research · 2019Article
- Characterization of Antigenic MHC-Class-I-Restricted T Cell Epitopes in the Glycoprotein of Ebolavirus.Cell reports · 2019Article
- Rapid selection and identification of functional CD8Nature communications · 2019Article
- Conserved peptide vaccine candidates containing multiple Ebola nucleoprotein epitopes display interactions with diverse HLA molecules.Medical microbiology and immunology · 2019Article
- Peptide Super-Agonist Enhances T-Cell Responses to Melanoma.Frontiers in immunology · 2019Article
- Peptide-MHC Class I Tetramers Can Fail To Detect Relevant Functional T Cell Clonotypes and Underestimate Antigen-Reactive T Cell Populations.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Hemophilia A inhibitor treatment: the promise of engineered T-cell therapy.Translational research : the journal of laboratory and clinical medicine · 2017Review
- Metabolic Adaptation of Human CD4Frontiers in immunology · 2017Article
- Article
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11 authors.
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Abstract
Isolation of peptide-specific T-cell clones is highly desirable for determining the role of T-cells in human disease, as well as for the development of therapies and diagnostics. However, generation of monoclonal T-cells with the required specificity is challenging and time-consuming. Here we describe a library-based strategy for the simple parallel detection and isolation of multiple peptide-specific human T-cell clones from CD8(+) or CD4(+) polyclonal T-cell populations. T-cells were first amplified by CD3/CD28 microbeads in a 96U-well library format, prior to screening for desired peptide recognition. T-cells from peptide-reactive wells were then subjected to cytokine-mediated enrichment followed by single-cell cloning, with the entire process from sample to validated clone taking as little as 6 weeks. Overall, T-cell libraries represent an efficient and relatively rapid tool for the generation of peptide-specific T-cell clones, with applications shown here in infectious disease (Epstein-Barr virus, influenza A, and Ebola virus), autoimmunity (type 1 diabetes) and cancer.
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