ArticleApplied and environmental microbiology2016
Improved Acetic Acid Resistance in Saccharomyces cerevisiae by Overexpression of the WHI2 Gene Identified through Inverse Metabolic Engineering.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Fermentation-associated compositional changes in hot-brewedFood science and biotechnology · 2026Article
- Bacillus alcalophilus RecJ (BaRecJ) can drive the adaptive evolution of S. cerevisiae.Biotechnology letters · 2025Article
- Development of a RobustMicroorganisms · 2024Article
- Towards universal synthetic heterotrophy using a metabolic coordinator.Metabolic engineering · 2023Article
- Prospecting and engineering yeasts for ethanol production under inhibitory conditions: an experimental design analysis.Bioprocess and biosystems engineering · 2023Article
- How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses.Current genetics · 2022Review
- Whole-Genome Transformation of Yeast Promotes Rare Host Mutations with a Single Causative SNP Enhancing Acetic Acid Tolerance.Molecular and cellular biology · 2022Article
- Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains.Biotechnology for biofuels and bioproducts · 2022Review
- Advances and prospects in metabolic engineering of Escherichia coli for L-tryptophan production.World journal of microbiology & biotechnology · 2022Review
- Identification of Kic1p and Cdc42p as Novel Targets to Engineer Yeast Acetic Acid Stress Tolerance.Frontiers in bioengineering and biotechnology · 2022Article
- Acetic acid stress in budding yeast: From molecular mechanisms to applications.Yeast (Chichester, England) · 2021Review
- Recent advances in improving metabolic robustness of microbial cell factories.Current opinion in biotechnology · 2020Review
- Metabolic Changes Induced by Deletion of Transcriptional RegulatorMicroorganisms · 2020Article
- The Whi2p-Psr1p/Psr2p complex regulates interference competition and expansion of cells with competitive advantage in yeast colonies.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Feasibility study of on-site solid-state enzyme production byBiotechnology for biofuels · 2020Article
- Polygenic analysis of very high acetic acid tolerance in the yeastBiotechnology for biofuels · 2020Article
- Unique genetic basis of the distinct antibiotic potency of high acetic acid production in the probiotic yeastGenome research · 2019Article
- Enhanced acetic acid stress tolerance and ethanol production inBiotechnology for biofuels · 2019Article
- Evaluation of divergent yeast genera for fermentation-associated stresses and identification of a robust sugarcane distillery waste isolateBiotechnology for biofuels · 2019Article
- Single and combined effects of acetic acid, furfural, and sugars on the growth of the pentose-fermenting yeast3 Biotech · 2018Article
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3 authors.
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Abstract
Development of acetic acid-resistant Saccharomyces cerevisiae is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge due to limited information on effective genetic perturbation targets for improving acetic acid resistance in the yeast. This study employed a genomic-library-based inverse metabolic engineering approach to successfully identify a novel gene target, WHI2 (encoding a cytoplasmatic globular scaffold protein), which elicited improved acetic acid resistance in S. cerevisiae. Overexpression of WHI2 significantly improved glucose and/or xylose fermentation under acetic acid stress in engineered yeast. The WHI2-overexpressing strain had 5-times-higher specific ethanol productivity than the control in glucose fermentation with acetic acid. Analysis of the expression of WHI2 gene products (including protein and transcript) determined that acetic acid induced endogenous expression of Whi2 in S. cerevisiae. Meanwhile, the whi2Δ mutant strain had substantially higher susceptibility to acetic acid than the wild type, suggesting the important role of Whi2 in the acetic acid response in S. cerevisiae. Additionally, overexpression of WHI2 and of a cognate phosphatase gene, PSR1, had a synergistic effect in improving acetic acid resistance, suggesting that Whi2 might function in combination with Psr1 to elicit the acetic acid resistance mechanism. These results improve our understanding of the yeast response to acetic acid stress and provide a new strategy to breed acetic acid-resistant yeast strains for renewable biofuel production.
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