ArticleApplied and environmental microbiology2016
Thermal Inactivation of Enteric Viruses and Bioaccumulation of Enteric Foodborne Viruses in Live Oysters (Crassostrea virginica).
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- An RTCA-based assay as an innovative approach for thermal inactivation studies of hepatitis A virus.Applied and environmental microbiology · 2026Article
- Research Progress on Biological Accumulation, Detection and Inactivation Technologies of Norovirus in Oysters.Foods (Basel, Switzerland) · 2023Review
- Promise and perils of paper-based point-of-care nucleic acid detection for endemic and pandemic pathogens.Lab on a chip · 2023Review
- Temperature Dependent Depuration of Norovirus GII and Tulane Virus from Oysters (Crassostrea gigas).Food and environmental virology · 2023Article
- Low-Cost UVBot Using SLAM to Mitigate the Spread of Noroviruses in Occupational Spaces.Sensors (Basel, Switzerland) · 2022Article
- Inactivation Mechanism and Efficacy of Grape Seed Extract for Human Norovirus Surrogate.Applied and environmental microbiology · 2022Article
- Inactivation of Murine Norovirus Suspended in Organic Matter Simulating Actual Conditions of Viral Contamination.Food and environmental virology · 2021Article
- Standard hospital blanket warming cabinets can be utilized for complete moist heat SARS-CoV2 inactivation of contaminated N95 masks for re-use.Scientific reports · 2021Article
- Quantitative Real-Time PCR and Digital PCR to Evaluate Residual Quantity of HAV in Experimentally Depurated Mussels.Food and environmental virology · 2021Article
- Occurrence of Human Enteric Viruses in Water Sources and Shellfish: A Focus on Africa.Food and environmental virology · 2021Review
- Thermal Inactivation of Hepatitis A Virus, Noroviruses, and Simian Rotavirus in Cows' Milk.Food and environmental virology · 2020Article
- Occurrence, fates and potential treatment approaches for removal of viruses from wastewater: A review with emphasis on SARS-CoV-2.Journal of environmental chemical engineering · 2020Review
- Accumulation and Depuration Kinetics of Rotavirus in Mussels Experimentally Contaminated.Food and environmental virology · 2020Article
- UV Inactivation of Rotavirus and Tulane Virus Targets Different Components of the Virions.Applied and environmental microbiology · 2020Article
- Decontamination of N95 masks for re-use employing 7 widely available sterilization methods.PloS one · 2020Article
- Assessment of the Applicability of Capsid-Integrity Assays for Detecting Infectious Norovirus Inactivated by Heat or UV Irradiation.Food and environmental virology · 2019Article
- Detection of Hepatitis A Virus and Other Enteric Viruses in Shellfish Collected in the Gulf of Naples, Italy.International journal of environmental research and public health · 2019Article
- Weekly Variation of Rotavirus A Concentrations in Sewage and Oysters in Japan, 2014-2016.Pathogens (Basel, Switzerland) · 2019Article
- Specific Interactions between Human Norovirus and Environmental Matrices: Effects on the Virus Ecology.Viruses · 2019Review
- Development of a Real-Time Cell Analysis (RTCA) Method as a Fast and Accurate Method for Detecting Infectious Particles of the Adapted Strain of Hepatitis A Virus.Frontiers in cellular and infection microbiology · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human enteric viruses are among the main causative agents of shellfish-associated outbreaks. In this study, the kinetics of viral bioaccumulation in live oysters and the heat stabilities of the predominant enteric viruses were determined both in tissue culture and in oyster tissues. A human norovirus (HuNoV) GII.4 strain, HuNoV surrogates (murine norovirus [MNV-1], Tulane virus [TV]), hepatitis A virus (HAV), and human rotavirus (RV) bioaccumulated to high titers within oyster tissues, with different patterns of bioaccumulation for the different viruses. We tested the thermal stability of each virus at 62, 72, and 80°C in culture medium. The viruses can be ranked from the most heat resistant to the least stable as follows: HAV, RV, TV, MNV-1. In addition, we found that oyster tissues provided protection to the viruses during heat treatment. To decipher the mechanism underlying viral inactivation by heat, purified TV was treated at 80°C for increasing time intervals. It was found that the integrity of the viral capsid was disrupted, whereas viral genomic RNA remained intact. Interestingly, heat treatment leading to complete loss of TV infectivity was not sufficient to completely disrupt the receptor binding activity of TV, as determined by the porcine gastric mucin-magnetic bead binding assay. Similarly, HuNoV virus-like particles (VLPs) and a HuNoV GII.4 strain retained some receptor binding ability following heat treatment. Although foodborne viruses have variable heat stability, 80°C for >6 min was sufficient to completely inactivate enteric viruses in oysters, with the exception of HAV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.