Evidence map›Paper›PMID 26825681›Full record

ArticleCytotechnology2016

Epithelial cells from oral mucosa: How to cultivate them?

F B Russo, G C Pignatari, I R Fernandes, J L R M Dias, P C B Beltrão-Braga

Open access · greenAbstract read
In one paragraph

Article in Cytotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

F B RussoStem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil. fabielerusso@gmail.com.
G C PignatariStem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil.
I R FernandesStem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil.
J L R M DiasStem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil.
P C B Beltrão-BragaStem Cell Lab, Department of Surgery, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Av., São Paulo, 05508-270, Brazil.
Universidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Epithelial cells from oral mucosa (EOM) are responsible for important functions, like the primary protection of oral mucosa against external aggressions building a mechanical barrier against microorganisms, mechanical damage, toxic material, thermal regulation and secretion of different classes of inflammatory mediators. EOM could be an interesting tool for cellular and molecular biology research. Usually, EOM are collected by a painful and invasive process. In this study, we propose an alternative method to cultivate EOM collected by non-invasive scraping method of oral mucosa. Papanicolaou staining showed mainly two kinds of epithelial cell population after EOM scraping. As result of the five culture methods tested here, our results revealed that the EOM were successfully cultured on a murine feeder layer. In addition, EOM could be frozen and thawed, without morphology changes and loss of viability. Our findings suggest that EOM can be considered as a good cell source for many purposes, such as genetic studies, diagnosis and cell therapy.

Indexed as

Cell cultureEpithelial cellsEpithelial cells from oral mucosaNon-invasive methodOral mucosa

Identifiers

PMID26825681
PMCPMC5023582
OpenAlexW2286566150

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.