ArticleJournal of bone and mineral metabolism2017
In vivo expression and regulation of genes associated with vascularization during early response of sutures to tensile force.
Article in Journal of bone and mineral metabolism, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Association of bevacizumab and stroke in ovarian cancer: a systematic review and meta-analysis.Frontiers in neuroscience · 2023Pooled it
- Spatiotemporal dynamics and regulation of suture morphology and collagen remodeling during trans-sutural distraction osteogenesis.NPJ Regenerative medicine · 2026Article
- Regional variability in craniofacial stiffness: a study in normal and Crouzon mice during postnatal development.Biomechanics and modeling in mechanobiology · 2025Article
- Cyclic loading failed to promote growth in a pig model of midfacial hypoplasia.Journal of anatomy · 2024Article
- Distraction force promotes the osteogenic differentiation of Gli1Stem cell research & therapy · 2024Article
- Expansion of the sagittal suture induces proliferation of skeletal stem cells and sustains endogenous calvarial bone regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Allyl Isothiocyanate (AITC) Induces Apoptotic Cell Death In Vitro and Exhibits Anti-Tumor Activity in a Human Glioblastoma GBM8401/International journal of molecular sciences · 2022Article
- Suture Cells in a Mechanical Stretching Niche: Critical Contributors to Trans-sutural Distraction Osteogenesis.Calcified tissue international · 2022Review
- Polarized M2 macrophages induced by mechanical stretching modulate bone regeneration of the craniofacial suture for midfacial hypoplasia treatment.Cell and tissue research · 2021Article
- Connective tissue growth factor promotes chemotaxis of preosteoblasts through integrin α5 and Ras during tensile force-induced intramembranous osteogenesis.Scientific reports · 2021Article
- Hydroxyapatite Nanoparticles Facilitate Osteoblast Differentiation and Bone Formation Within Sagittal Suture During Expansion in Rats.Drug design, development and therapy · 2021Article
- Integrated Transcriptome and Network Analysis Reveals Spatiotemporal Dynamics of Calvarial Suturogenesis.Cell reports · 2020Article
- Methionine Enkephalin Suppresses Osteocyte Apoptosis Induced by Compressive Force through Regulation of Nuclear Translocation of NFATc1.JBMR plus · 2020Article
- VEGF and SEMA4D have synergistic effects on the promotion of angiogenesis in epithelial ovarian cancer.Cellular & molecular biology letters · 2018Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sutures are fibrous tissues that connect bones in craniofacial skeletal complexes. Cranio- and dentofacial skeletal deformities in infant and adolescent patients can be treated by applying tensile force to sutures to induce sutural bone formation. The early gene expression induced by mechanical stress is essential for bone formation in long bones; however, early gene expression during sutural bone formation induced by tensile force is poorly characterized. In vivo studies are essential to evaluate molecular responses to mechanical stresses in heterogeneous cell populations, such as sutures. In this paper we examined in vivo early gene expression and the underlying regulatory mechanism for this expression in tensile-force-applied cranial sutures, focusing on genes involved in vascularization. Tensile force upregulated expression of vascular factors, such as vascular endothelial growth factor (Vegf) and endothelial cell markers, in sutures within 3 h. The expression of connective tissue growth factor (Ctgf) and Rho-associated coiled-coil containing protein kinase 2 (Rock2) was also upregulated by tensile force. A CTGF-neutralizing antibody and the ROCK inhibitor, Y-27632, abolished tensile-force-induced Vegf expression. Moreover, tensile force activated extracellular signal-related kinase 1/2 (ERK1/2) signaling in sagittal sutures, and the ERK1/2 inhibitor, U0126, partially inhibited tensile-force-induced Ctgf expression. These results indicate that tensile force induces in vivo gene expression associated with vascularization early in tensile-force-induced sutural bone formation. Moreover, the early induction of Vegf gene expression is regulated by CTGF and ROCK2.
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