ArticlePloS one2016
Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- MiniFAST: a sensitive and fast miniaturized microscope forNeurophotonics · 2026Article
- Principles of microscopy for ophthalmologists.Eye (London, England) · 2025Review
- Widefield polarization-resolved second harmonic generation imaging of entire thyroid nodule sections for the detection of capsular invasion.Biomedical optics express · 2024Article
- Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators.Journal of biomedical optics · 2022Article
- Challenges and advances in optical 3D mesoscale imaging.Journal of microscopy · 2022Article
- FastBiomedical optics express · 2020Article
- Fluorescent bicolour sensor for low-background neutrinoless double β decay experiments.Nature · 2020Article
- Fast Optical Sectioning for Widefield Fluorescence Mesoscopy with the Mesolens based on HiLo Microscopy.Scientific reports · 2018Article
- A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric CaJournal of microscopy · 2018Article
- Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synaptically-driven Ca(2+) events in primary rat hippocampal neurone cultures loaded with the fluorescent Ca(2+) indicator Fluo-4 AM, we have observed greatly reduced photo-bleaching in comparison with single-photon excitation. This method, which requires no costly additions to the microscope, promises to be useful for work where high time-resolution is required.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.