ArticleMolecular reproduction and development2016
Glycine supplementation in vitro enhances porcine preimplantation embryo cell number and decreases apoptosis but does not lead to live births.
Article in Molecular reproduction and development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Glycine as a Metabolic Regulator of Reproductive Function in Livestock: From Gametes to Early Embryos.Animals : an open access journal from MDPI · 2026Review
- The role of metabolic reprogramming in oocyte development: research progress and prospects.Frontiers in reproductive health · 2026Review
- Stage-dependent changes in culture medium osmolality promote porcine oocyte maturationFrontiers in cell and developmental biology · 2025Article
- Treatment of Donor Cells with Oxidative Phosphorylation Inhibitor CPI Enhances Porcine Cloned Embryo Development.Animals : an open access journal from MDPI · 2024Article
- Glycine regulates lipid peroxidation promoting porcine oocyte maturation and early embryonic development.Journal of animal science · 2023Article
- Chloride channel accessory 1 gene deficiency causes selective loss of mucus production in a new pig model.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Supplementation of fetal bovine serum increased the quality ofJournal of advanced veterinary and animal research · 2021Article
- Review
- Glutaminolysis is involved in the activation of mTORC1 in in vitro-produced porcine embryos.Molecular reproduction and development · 2021Article
- Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production.Scientific reports · 2021Article
- Glycine ameliorates mitochondrial dysfunction caused by ABT-199 in porcine oocytes.Journal of animal science · 2021Article
- Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion.Journal of clinical medicine · 2020Article
- [Protective effect of serine methyltransferase against hepatic ischemia-reperfusion injury in mice].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2020Article
- Generation of human endothelium in pig embryos deficient in ETV2.Nature biotechnology · 2020Article
- Metabolic Profiling in Blastocoel Fluid and Blood Plasma of Diabetic Rabbits.International journal of molecular sciences · 2020Article
- Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle.International journal of molecular sciences · 2019Article
- Applications of omics and nanotechnology to improve pig embryo production in vitro.Molecular reproduction and development · 2019Review
- Glutamine supplementation enhances development of in vitro-produced porcine embryos and increases leucine consumption from the medium.Biology of reproduction · 2018Article
- Glycine treatment enhances developmental potential of porcine oocytes and early embryos by inhibiting apoptosis.Journal of animal science · 2018Article
- Pharmacologic treatment of donor cells induced to have a Warburg effect-like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs.Molecular reproduction and development · 2018Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Most in vitro culture conditions are less-than-optimal for embryo development. Here, we used a transcriptional-profiling database to identify culture-induced differences in gene expression in porcine blastocysts compared to in vivo-produced counterparts. Genes involved in glycine transport (SLC6A9), glycine metabolism (GLDC, GCSH, DLD, and AMT), and serine metabolism (PSAT1, PSPH, and PHGDH) were differentially expressed. Addition of 10 mM glycine to the culture medium (currently containing 0.1 mM) reduced the abundance of SLC6A9 transcript and increased total cell number, primarily in the trophectoderm lineage (P = 0.003); this was likely by decreasing the percentage of apoptotic nuclei. As serine and glycine can be reversibly metabolized by serine hydroxymethyltransferase 2 (SHMT2), we assessed the abundance of SHMT2 transcript as well as its functional role by inhibiting it with aminomethylphosphonic acid (AMPA), a glycine analog, during in vitro culture. Both AMPA supplementation and elevated glycine decreased the mRNA abundance of SHMT2 and tumor protein p53 (TP53), which is activated in response to cellular stress, compared to controls (P ≤ 0.02). On the other hand, mitochondrial activity of blastocysts, mtDNA copy number, and abundance of mitochondria-related transcripts did not differ between control and 10 mM glycine culture conditions. Despite improvements to these metrics of blastocyst quality, transfer of embryos cultured in 10 mM glycine did not result in pregnancy whereas the transfer of in vitro-produced embryos cultured in control medium yielded live births. Mol. Reprod. Dev. 83: 246-258, 2016. © 2016 The Authors.
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