ArticlePloS one2016
Rapid Identification of Candidate Genes for Seed Weight Using the SLAF-Seq Method in Brassica napus.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 85 citations in OpenAlex.
- Article
- Fine mapping and candidate gene analysis of the major QTL qSW-A03 for seed weight in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- SNP-based linkage mapping reveals novel quantitative trait loci for yield traits in noug (Frontiers in plant science · 2025Article
- Combined BSA-seq and RNA-seq approaches reveal candidate genes associated with seed weight inFrontiers in plant science · 2025Article
- Identification of molecular markers and candidate regions associated with grain number per spike in Pubing3228 using SLAF-BSA.Frontiers in plant science · 2024Article
- Review
- Genome-Wide Association Studies of Salt Tolerance at the Seed Germination Stage and Yield-Related Traits inInternational journal of molecular sciences · 2022Article
- Allelically and Differentially Expressed Genes After Infection of Edwardsiella ictaluri in Channel Catfish as Determined by Bulk Segregant RNA-Seq.Marine biotechnology (New York, N.Y.) · 2022Article
- A preliminary mapping of QTLFrontiers in plant science · 2022Article
- Phylogeographical Pattern and Population Evolution History of IndigenousFrontiers in plant science · 2022Article
- Transcriptomic analysis of rapeseed (Frontiers in plant science · 2022Article
- Physical mapping and InDel marker development for the restorer gene RfBMC genomics · 2021Article
- Genome-Wide Association Study of Root and Shoot Related Traits in Spring Soybean (Frontiers in plant science · 2021Article
- Transcriptomics Analysis and Re-sequencing Reveal the Mechanism Underlying the Thermotolerance of an Artificial Selection Population of the Pacific Oyster.Frontiers in physiology · 2021Article
- Investigation of Thermomorphogenesis-Related Genes for a Multi-Silique Trait inFrontiers in genetics · 2021Article
- A mutation in the intron splice acceptor site of a GA3ox gene confers dwarf architecture in watermelon (Citrullus lanatus L.).Scientific reports · 2020Article
- Fine Mapping of a Locus Underlying the Ectopic Blade-Like Outgrowths on Leaf and Screening Its Candidate Genes in Rapeseed (Frontiers in plant science · 2020Article
- Identification of a Major QTL (International journal of molecular sciences · 2019Article
- Mapping Gene Markers for Apple Fruit Ring Rot Disease Resistance Using a Multi-omics Approach.G3 (Bethesda, Md.) · 2019Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seed weight is a critical and direct trait for oilseed crop seed yield. Understanding its genetic mechanism is of great importance for yield improvement in Brassica napus breeding. Two hundred and fifty doubled haploid lines derived by microspore culture were developed from a cross between a large-seed line G-42 and a small-seed line 7-9. According to the 1000-seed weight (TSW) data, the individual DNA of the heaviest 46 lines and the lightest 47 lines were respectively selected to establish two bulked DNA pools. A new high-throughput sequencing technology, Specific Locus Amplified Fragment Sequencing (SLAF-seq), was used to identify candidate genes of TSW in association analysis combined with bulked segregant analysis (BSA). A total of 1,933 high quality polymorphic SLAF markers were developed and 4 associated markers of TSW were procured. A hot region of ~0.58 Mb at nucleotides 25,401,885-25,985,931 on ChrA09 containing 91 candidate genes was identified as tightly associated with the TSW trait. From annotation information, four genes (GSBRNA2T00037136001, GSBRNA2T00037157001, GSBRNA2T00037129001 and GSBRNA2T00069389001) might be interesting candidate genes that are highly related to seed weight.
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