ArticleTranslation (Austin, Tex.)2013
PUNCH-P for global translatome profiling: Methodology, insights and comparison to other techniques.
Article in Translation (Austin, Tex.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Clu1/Clu form mitochondria-associated granules upon metabolic transitions and regulate mitochondrial protein translation via ribosome interactions.PLoS genetics · 2025Article
- Pervasiveness of Microprotein Function AmongstCells · 2024Article
- Effective extraction of polyribosomes exposes gene expression strategies in primary astrocytes.Nucleic acids research · 2023Article
- Loss of 4E-BPs prevents the hindlimb immobilization-induced decrease in protein synthesis in skeletal muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γ.Inflammation · 2022Article
- Meeting the complexity of plant nutrient metabolism with multi-omics approaches.Journal of experimental botany · 2021Article
- Article
- Assessment of Streptavidin Bead Binding Capacity to Improve Quality of Streptavidin-based Enrichment Studies.Journal of proteome research · 2021Article
- Spatiotemporal Insights Into RNA-Organelle Interactions in Neurons.Frontiers in cell and developmental biology · 2021Review
- Involvement of the eIF2α Kinase GCN2 in UV-B Responses.Frontiers in plant science · 2019Article
- Analysis of the translatome in solid tumors using polyribosome profiling/RNA-Seq.Journal of biological methods · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of mRNA translation is a major modulator of gene expression, allowing cells to fine tune protein levels during growth and differentiation and in response to physiological signals and environmental changes. Mass-spectrometry and RNA-sequencing methods now enable global profiling of the translatome, but these still involve significant analytical and economical limitations. We developed a novel system-wide proteomic approach for direct monitoring of translation, termed PUromycin-associated Nascent CHain Proteomics (PUNCH-P), which is based on the recovery of ribosome-nascent chain complexes from cells or tissues followed by incorporation of biotinylated puromycin into newly-synthesized proteins. Biotinylated proteins are then purified by streptavidin and analyzed by mass-spectrometry. Here we present an overview of PUNCH-P, describe other methodologies for global translatome profiling (pSILAC, BONCAT, TRAP/Ribo-tag, Ribo-seq) and provide conceptual comparisons between these methods. We also show how PUNCH-P data can be combined with mRNA measurements to determine relative translation efficiency for specific mRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.