ArticleInternational journal of clinical and experimental pathology2015
Development of a new real-time PCR system for simultaneous detection of bacteria and fungi in pathological samples.
Article in International journal of clinical and experimental pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Cross-kingdom pathogen detection via duplex universal PCR and high-resolution melt.Biosensors & bioelectronics · 2025Article
- Highly accurate measurement of the relative abundance of oral pathogenic bacteria using colony-forming unit-based qPCR.Journal of periodontal & implant science · 2024Article
- Revolutionizing chronic endometritis diagnosis: real-time polymerase chain reaction unveils microbial pathogens in Indian women with abnormal bleeding and reproductive challenges.AJOG global reports · 2024Article
- Unified metagenomic method for rapid detection of microorganisms in clinical samples.Communications medicine · 2024Article
- Draft genome sequences of fourMicrobiology resource announcements · 2024Article
- Bacterial colonization of the upper airways of children positive and negative for SARS-CoV-2 during the COVID-19 pandemic.BMC infectious diseases · 2022Article
- Comparison Approach for Identifying Missed Invasive Fungal Infections in Formalin-Fixed, Paraffin-Embedded Autopsy Specimens.Journal of fungi (Basel, Switzerland) · 2022Article
- An atypical case of Kawasaki disease with severe pneumonia in a neonate.BMC pediatrics · 2022Article
- Evaluating the potential for respiratory metagenomics to improve treatment of secondary infection and detection of nosocomial transmission on expanded COVID-19 intensive care units.Genome medicine · 2021Article
- Lung Pathology of Mutually Exclusive Co-infection with SARS-CoV-2 and Streptococcus pneumoniae.Emerging infectious diseases · 2021Article
- Gestational Psittacosis With Secondary Hemophagocytic Syndrome: A Case Report and Literature Review.Frontiers in medicine · 2021Article
- Effect of Silver Diamine Fluoride on Reducing Candida albicans Adhesion on Dentine.Mycopathologia · 2020Article
- MiR-21 Is Remotely Governed by the Commensal Bacteria and Impairs Anti-TB Immunity by Down-Regulating IFN-γ.Frontiers in microbiology · 2020Article
- Article
- A lung abscess caused by secondary syphilis - the utility of polymerase chain reaction techniques in transbronchial biopsy: a case report.BMC infectious diseases · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel system for simultaneous detection of pathogenic bacteria and fungi in pathological samples was developed using a real-time polymerase chain reaction (PCR) system. This system, designated the "multi-microbial real-time PCR", has the potential to simultaneously detect 68 bacterial and 9 fungal species in a 96-well plate format. All probe-primer sets were designed to produce amplicons smaller than 210 bp using formalin-fixed paraffin-embedded samples as input. The specificity and sensitivity of each probe-primer set were tested against DNA extracted from pure cultures of specific pathogens. The multi-microbial real-time PCR system revealed profiles of microorganism infection in lung samples collected at autopsy from 10 patients with acquired immunodeficiency syndrome. Staphylococcus aureus was the most common microbe detected (n=8), but with low copy numbers. High copy numbers of Pseudomonas aeruginosa were detected in the lung samples with abscess (n=6). Enterococcus faecium (n=6), Elizabethkingia meningoseptica (n=4), and Candida albicans (n=4) were also frequently detected. In addition, a latent infection of Mycobacterium tuberculosis was detected in one case of pneumonia. In conclusion, this multi-microbial real-time PCR system can be useful for detecting bacteria and fungi in pathological specimens from patients with uncertain diagnoses.
Indexed as
Identifiers
26823918PMC4713704What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.