ReviewPlant methods2016
Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas.
Review in Plant methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 153 citations in OpenAlex.
- Impact ofBiotech (Basel (Switzerland)) · 2025Article
- Putting CRISPR-Cas system in action: a golden window for efficient and precise genome editing for crop improvement.GM crops & food · 2023Review
- CRISPR/Cas9-gene editing approaches in plant breeding.GM crops & food · 2023Review
- Transformative Approaches for Sustainable Weed Management: The Power of Gene Drive and CRISPR-Cas9.Genes · 2023Review
- Accelerated Breeding forPlants (Basel, Switzerland) · 2023Review
- The heat is on: a simple method to increase genome editing efficiency in plants.BMC plant biology · 2022Article
- A New Approach to Develop Resistant Cultivars Against the Plant Pathogens: CRISPR Drives.Frontiers in plant science · 2022Article
- Application of CRISPR/Cas9 technology in wild apple (Malus sieverii) for paired sites gene editing.Plant methods · 2021Article
- CRISPR/Cas9-Mediated Genome Editing in Comfrey (Molecules (Basel, Switzerland) · 2021Article
- Advances in Genetic Engineering Technology and Its Application in the Industrial FungusFrontiers in microbiology · 2021Review
- Proxies of CRISPR/Cas9 Activity To Aid in the Identification of Mutagenized Arabidopsis Plants.G3 (Bethesda, Md.) · 2020Article
- Application of CRISPR/Cas for Diagnosis and Management of Viral Diseases of Banana.Frontiers in microbiology · 2020Review
- Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa.Frontiers in plant science · 2020Review
- A systematic analysis of apple root resistance traits toHorticulture research · 2020Review
- CRISPR/Cas9-mediated gfp gene inactivation in Arabidopsis suspension cells.Molecular biology reports · 2019Article
- CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective.Applied microbiology and biotechnology · 2019Review
- Construction of a One-Vector Multiplex CRISPR/Cas9 Editing System to Inhibit Nucleopolyhedrovirus Replication in Silkworms.Virologica Sinica · 2019Article
- Design of a bacterial speck resistant tomato by CRISPR/Cas9-mediated editing of SlJAZ2.Plant biotechnology journal · 2019Article
- Enhancing oil production inBiotechnology for biofuels · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The precise manipulation of plant genomes relies on the induction of DNA double-strand breaks by site-specific nucleases to initiate DNA repair reactions that are either based on non-homologous end joining (NHEJ) or homologous recombination (HR). Recently, the CRISPR/Cas system emerged as the most important tool for genome engineering due to its simple structure and its applicability to a wide range of organisms. Here, we review the current status of its various applications in plants, where it is used for the successful generation of stable mutations in a steadily growing number of species through NHEJ. Furthermore, tremendous progress in plant genome engineering by HR was obtained by the setup of replicon mediated and in planta gene targeting techniques. Finally, other complex approaches that rely on the induction of more than one DNA lesion at a time such as paired nickases to avoid off-site effects or controlled genomic deletions are beginning to be applied routinely.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.