Evidence map›Paper›PMID 26823661›Full record

ArticleInternational journal of plant genomics2015

Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae).

Dmitry V Politov, Maryana M Belokon, Yuri S Belokon, Tatyana A Polyakova, Anna V Shatokhina, Elena A Mudrik, Anna B Azarova, Mikhail V Filippov, Konstantin A Shestibratov

Abstract read
In one paragraph

Article in International journal of plant genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Molecular Identification and Karyological Analysis of a Rampant AspenInternational journal of plant genomics · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Dmitry V PolitovVavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russia.
Maryana M BelokonVavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russia.
Yuri S BelokonVavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russia.
Tatyana A PolyakovaVavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russia.
Anna V ShatokhinaVavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russia.
Elena A MudrikVavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Street 3, Moscow 119991, Russia.
Anna B AzarovaShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch, Pushchino 142290, Russia.
Mikhail V FilippovShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch, Pushchino 142290, Russia.
Konstantin A ShestibratovShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch, Pushchino 142290, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Testing systems for molecular identification of micropropagated elite aspen (Populus tremula L.) genotypes were developed on the base on microsatellite (SSR) loci. Out of 33 tested microsatellite loci, 14 were selected due to sustainable PCR amplification and substantial variability in elite clones of aspen aimed for establishment of fast-rotated forest plantations. All eight tested clones had different multilocus genotypes. Among 114 trees from three reference native stands located near the established plantations, 80 haplotypes were identified while some repeated genotypes were attributed to natural clones which appeared as a result of sprouting. The selected set of SSR markers showed reliable individual identification with low probability of appearance of identical aspen genotypes (a minimum of 4.8 · 10(-10) and 1 × 10(-4) for unrelated and related individuals, resp.). Case studies demonstrating practical applications of the test system are described including analysis of clonal structure and levels of genetic diversity in three natural aspen stands growing in the regions where plantations made of elite clones were established.

Identifiers

PMID26823661
PMCPMC4707373

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.