ArticlePersoonia2015
One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes.
Article in Persoonia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 228 papers.
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228 citing papers in PubMed.
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- Taxonomic revision of the genusPersoonia · 2026Article
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- Endophytic Fungi Associated with Seaweeds as Potential Producers of Antimicrobial Compounds.Marine biotechnology (New York, N.Y.) · 2026Article
- Limitations of Common Molecular Markers in Fungal Biodiversity Analysis and the Benefits of Their Synergistic Use.Molecular ecology resources · 2026Article
- Molecular Identification and RNA-Based Management of Fungal Plant Pathogens: From PCR to CRISPR/Cas9.International journal of molecular sciences · 2026Review
- Characterization and Virulence of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) Isolates from Ecologically Distinct Soils Against Spodoptera litura (Lepidoptera: Noctuidae).Journal of insect science (Online) · 2026Article
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- Assessment of antibacterial and anti-biofilm activities of marine fungal epiphytes associated with red and green algae of the Kenyan coast.Biotechnology notes (Amsterdam, Netherlands) · 2026Article
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- Advancing Yeast Identification Using High-Throughput DNA Barcode Data From a Curated Culture Collection.Molecular ecology resources · 2026Article
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- Phylogeny, taxonomy and geographic distribution of novel and known fungi with holoblastic-denticulate conidiogenesis inPersoonia · 2025Article
- Basidiomycete Yeasts of Wine Grapes and Their Potential Applications in Winemaking.Comprehensive reviews in food science and food safety · 2025Review
- Arthroderma guizhouense sp. nov., a Novel Geophilic Species from Guizhou, China.Mycopathologia · 2025Article
- Population, Physiological, and Genetic Insights into Carbendazim Resistance in Populations of the Phytopathogenic FungusJournal of fungi (Basel, Switzerland) · 2025Article
168 more citing papers are in PubMed but not listed here.
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63 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Amplification efficiencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across > 1 500 species (1 931 strains or specimens) and the outcomes of almost twenty thousand (19 577) polymerase chain reactions were evaluated. We tested several well-known primer pairs that amplify: i) sections of the nuclear ribosomal RNA gene large subunit (D1-D2 domains of 26/28S); ii) the complete internal transcribed spacer region (ITS1/2); iii) partial β -tubulin II (TUB2); iv) γ-actin (ACT); v) translation elongation factor 1-α (TEF1α); and vi) the second largest subunit of RNA-polymerase II (partial RPB2, section 5-6). Their PCR efficiencies were compared with novel candidate primers corresponding to: i) the fungal-specific translation elongation factor 3 (TEF3); ii) a small ribosomal protein necessary for t-RNA docking; iii) the 60S L10 (L1) RP; iv) DNA topoisomerase I (TOPI); v) phosphoglycerate kinase (PGK); vi) hypothetical protein LNS2; and vii) alternative sections of TEF1α. Results showed that several gene sections are accessible to universal primers (or primers universal for phyla) yielding a single PCR-product. Barcode gap and multi-dimensional scaling analyses revealed that some of the tested candidate markers have universal properties providing adequate infra- and inter-specific variation that make them attractive barcodes for species identification. Among these gene sections, a novel high fidelity primer pair for TEF1α, already widely used as a phylogenetic marker in mycology, has potential as a supplementary DNA barcode with superior resolution to ITS. Both TOPI and PGK show promise for the Ascomycota, while TOPI and LNS2 are attractive for the Pucciniomycotina, for which universal primers for ribosomal subunits often fail.
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