ArticleParasites & vectors2016
Characterisation of P-glycoprotein-9.1 in Haemonchus contortus.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- P-glycoproteins and amphids: a two-stage trajectory of ivermectin resistance in Caenorhabditis elegans.International journal for parasitology. Drugs and drug resistance · 2026Article
- Critical role of P-Glycoprotein-9 in ivermectin tolerance in nematodes.PLoS pathogens · 2026Article
- P-glycoprotein-9-mediated multidrug tolerance in Caenorhabditis elegans.Parasites & vectors · 2025Article
- Chromosomal genome assembly resolves drug resistance loci in the parasitic nematode Teladorsagia circumcincta.PLoS pathogens · 2025Article
- Article
- Expression of transporter genes in anthelmintic resistant isolates of Haemonchus contortus.Genetics and molecular biology · 2024Article
- Importance of ABC Transporters in the Survival of Parasitic Nematodes and the Prospect for the Development of Novel Control Strategies.Pathogens (Basel, Switzerland) · 2023Review
- Repertoire of P-glycoprotein drug transporters in the zoonotic nematode Toxocara canis.Scientific reports · 2023Article
- Comparative Metabolome Analyses of Ivermectin-Resistant and -Susceptible Strains ofAnimals : an open access journal from MDPI · 2023Article
- Advances in diagnosis and control of anthelmintic resistant gastrointestinal helminths infecting ruminants.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Transgenic Expression ofInternational journal of molecular sciences · 2022Article
- Sensitivity of Haemonchus contortus to anthelmintics using different in vitro screening assays: a comparative study.Parasites & vectors · 2022Article
- Pharyngeal Pumping and Tissue-Specific Transgenic P-Glycoprotein Expression Influence Macrocyclic Lactone Susceptibility inPharmaceuticals (Basel, Switzerland) · 2021Article
- The P-glycoprotein repertoire of the equine parasitic nematode Parascaris univalens.Scientific reports · 2020Article
- Sub-lethal doses of albendazole induce drug metabolizing enzymes and increase albendazole deactivation in Haemonchus contortus adults.Veterinary research · 2020Article
- Effects of cholesterol content on activity of P-glycoproteins and membrane physical state, and consequences for anthelmintic resistance in the nematode Haemonchus contortus.Parasite (Paris, France) · 2020Article
- Differential ABC transporter gene expression in adult Dirofilaria immitis males and females following in vitro treatment with ivermectin, doxycycline or a combination of both.Parasites & vectors · 2019Article
- Perspectives on the utility of moxidectin for the control of parasitic nematodes in the face of developing anthelmintic resistance.International journal for parasitology. Drugs and drug resistance · 2019Review
- Efficiency of Target Larvicides Is Conditioned by ABC-Mediated Transport in the Zoonotic Nematode Anisakis pegreffii.Antimicrobial agents and chemotherapy · 2018Article
- Structural model, functional modulation by ivermectin and tissue localization of Haemonchus contortus P-glycoprotein-13.International journal for parasitology. Drugs and drug resistance · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe existence nematodes of veterinary importance such as Haemonchus contortus resistant to anthelmintic drugs, including the macrocyclic lactones, has become a major concern in animal health. Macrocyclic lactone resistance in H. contortus seems to be multigenic including the active efflux of these drugs by P-glycoproteins, members of the ABC transporter family, present in this parasite. The goals of the present work were to determine the activity of H. contortus P-glycoprotein 9.1 (Hco-PGP-9.1) and its interaction with the avermectins, ivermectin, abamectin, and also the milbemycin, moxidectin. Additionally, the localisation of Hco-PGP-9.1 was sought in adult worms.
methodsHco-Pgp-9.1 was cloned and expressed in mammalian cells and its expression profile was determined at the transcriptional and protein level by qRT-PCR and Western-blot, respectively. The nematode transport activity was assessed using the tracer dye Rhodamine 123. A ligand competition assay between different macrocyclic lactones and Rhodamine 123 was used to establish whether or not there was interaction between Hco-PGP-9.1 and the avermectins (abamectin and ivermectin) or moxidectin. In addition, immunostaining was carried out to localise Hco-PGP-9.1 expression in the transgenic cells and in adult female parasites.
resultsHco-PGP-9.1 was expressed in the cell membrane of the transfected host cells and was able to extrude Rhodamine 123. Ivermectin and abamectin, but not moxidectin, had a pronounced inhibitory effect on the ability of Hco-PGP-9.1 to transport Rhodamine 123. Antibodies raised against Hco-PGP-9.1 epitopes localised to the uterus of adult female H. contortus.
conclusionsThese results suggest a strong interaction of the avermectins with Hco-PGP-9.1. However, possibly due to its physico-chemical properties, moxidectin had markedly less effect on Hco-PGP-9.1. Because of the greater interaction of the avermectins than moxidectin with this transporter, it is more likely to contribute to avermectin resistance than to moxidectin resistance in H. contortus. Possible over expression of Hco-PGP-9.1 in the female reproductive system in resistant worms could reduce paralysis of the uterus by macrocyclic lactones, allowing continued egg release in drug challenged resistant worms.
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