ArticleScientific reports2016
Direct flow cytometry measurements reveal a fine-tuning of symbiotic cell dynamics according to the host developmental needs in aphid symbiosis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 71 citations in OpenAlex.
- How do host age and nutrition affect density regulation of obligate versus facultative bacterial symbionts? Insights from the tsetse fly.ISME communications · 2025Article
- Novel Aphid-Repellent Fiber Mats Based on Poly(lactic acid)-Containing Ionic Liquids.ACS omega · 2024Article
- Development, feeding, and sex shape the relative quantity of the nutritional obligatory symbiontFrontiers in microbiology · 2024Article
- The nutritional dimension of facultative bacterial symbiosis in aphids: Current status and methodological considerations for future research.Current research in insect science · 2024Review
- A theoretical model for host-controlled regulation of symbiont density.Journal of evolutionary biology · 2023Article
- Seasonal wild dance of dual endosymbionts in the pear psyllid Cacopsylla pyricola (Hemiptera: Psylloidea).Scientific reports · 2023Article
- Subcellular Niche Segregation of Co-Obligate Symbionts in Whiteflies.Microbiology spectrum · 2023Article
- Secondary symbionts affect aphid fitness and the titer of primary symbiont.Frontiers in plant science · 2023Article
- The Di-Symbiotic Systems in the AphidsMicroorganisms · 2022Article
- Ploidy dynamics in aphid host cells harboring bacterial symbionts.Scientific reports · 2022Article
- Bacteriocyte plasticity in pea aphids facing amino acid stress or starvation during development.Frontiers in physiology · 2022Article
- Evolution and ontogeny of bacteriocytes in insects.Frontiers in physiology · 2022Review
- SymbiQuant: A Machine Learning Object Detection Tool for Polyploid Independent Estimates of Endosymbiont Population Size.Frontiers in microbiology · 2022Article
- Insect-host control of obligate, intracellular symbiont density.Proceedings. Biological sciences · 2021Article
- The boom and bust of the aphid's essential amino acid metabolism across nymphal development.G3 (Bethesda, Md.) · 2021Article
- Strong within-host selection in a maternally inherited obligate symbiont:Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Adaptive Variation ofACS omega · 2021Article
- Sustainable laser-based technology for insect pest control.Scientific reports · 2021Article
- Microbiome Structure of the AphidFrontiers in microbiology · 2021Article
- The Bacterial Flora Associated with the Polyphagous Aphid Aphis gossypii Glover (Hemiptera: Aphididae) Is Strongly Affected by Host Plants.Microbial ecology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endosymbiotic associations constitute a driving force in the ecological and evolutionary diversification of metazoan organisms. Little is known about whether and how symbiotic cells are coordinated according to host physiology. Here, we use the nutritional symbiosis between the insect pest, Acyrthosiphon pisum, and its obligate symbiont, Buchnera aphidicola, as a model system. We have developed a novel approach for unculturable bacteria, based on flow cytometry, and used this method to estimate the absolute numbers of symbionts at key stages of aphid life. The endosymbiont population increases exponentially throughout nymphal development, showing a growing rate which has never been characterized by indirect molecular techniques. Using histology and imaging techniques, we have shown that the endosymbiont-bearing cells (bacteriocytes) increase significantly in number and size during the nymphal development, and clustering in the insect abdomen. Once adulthood is reached and the laying period has begun, the dynamics of symbiont and host cells is reversed: the number of endosymbionts decreases progressively and the bacteriocyte structure degenerates during insect aging. In summary, these results show a coordination of the cellular dynamics between bacteriocytes and primary symbionts and reveal a fine-tuning of aphid symbiotic cells to the nutritional demand imposed by the host physiology throughout development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.