Evidence map›Paper›PMID 26822068›Full record

ArticleTraffic (Copenhagen, Denmark)2016

Measuring Exocytosis Rate Using Corrected Fluorescence Recovery After Photoconversion.

Nan Luo, An Yan, Zhenbiao Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Traffic (Copenhagen, Denmark), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Review
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  6. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nan LuoCenter for Plant Cell Biology, Institute of Integrated Genome Biology, University of California, Riverside, CA, USA.
An YanCenter for Plant Cell Biology, Institute of Integrated Genome Biology, University of California, Riverside, CA, USA.
Zhenbiao YangCenter for Plant Cell Biology, Institute of Integrated Genome Biology, University of California, Riverside, CA, USA.

Funding

Request for An Integrated Confocal-Atomic Force Microscopy SystemR01GM100130 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI CUI, YONGTAO · 2012 to 2020
$1.9M
NIGMS NIH HHS R01 GM100130
6 · The paper itself

Abstract

Exocytosis plays crucial roles in regulating the distribution and function of plasma membrane (PM) and extracellular matrix proteins. However, measuring the exocytosis rate of a specific protein by conventional methods is very difficult because of exocytosis-independent trafficking such as endocytosis, which also affects membrane protein distribution. Here, we describe a novel method, corrected fluorescence recovery after photoconversion, in which exocytosis-dependent and -independent trafficking events are measured simultaneously to accurately determine exocytosis rate. In this method, the protein-of-interest is tagged with Dendra2, a green-to-red photoconvertible fluorescent protein. Following the photoconversion of PM-localized Dendra2, both the recovery of the green signal and the changes in the photoconverted red signal are measured, and the rate of exocytosis is calculated from the changing rates of these two signals.

Indexed as

ExocytosisLightArabidopsisFluorescenceMicroscopy, Confocalconfocal microscopyexocytosisfluorescence recovery after photobleachingmembrane proteinsphotoconversionphotoconvertible fluorescent protein

Identifiers

PMID26822068
PMCPMC7513930

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.