ArticleThe Journal of reproduction and development2016
Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells.
Article in The Journal of reproduction and development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Scoping review on the embryonic developmental potential of tripronuclear zygotes and their use for clinical and research purposes.Journal of assisted reproduction and genetics · 2025Article
- Comparison of Four Protocols for In Vitro Differentiation of Human Embryonic Stem Cells into Trophoblast Lineages by BMP4 and Dual Inhibition of Activin/Nodal and FGF2 Signaling.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Generation, genomic characterization, and differentiation of triploid human embryonic stem cells.Stem cell reports · 2023Article
- Review
- Derivation of human triploid trophoblast stem cells.Journal of assisted reproduction and genetics · 2022Article
- Mesenchymal Stem/Stromal-Like Cells from Diploid and Triploid Human Embryonic Stem Cells Display Different Gene Expression Profiles.Iranian biomedical journal · 2021Article
- Specification of trophoblast from embryonic stem cells exposed to BMP4.Biology of reproduction · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Because the diploid human embryonic stem cells (hESCs) can be successfully derived from tripronuclear zygotes thus, they can serve as an alternative source of derivation of normal karyotype hESC lines. The aim of the present study was to compare the pluripotency and trophoblast differentiation ability of hESCs derived from tripronuclear zygotes and diploid hESCs. In the present study, a total of 20 tripronuclear zygotes were cultured; 8 zygotes developed to the blastocyst stage and 1 hESC line was generated. Unlike the previous studies, chromosomal correction of tripronuclear zygotes during derivation of hESCs did not occur. The established line carries 3 sets of chromosomes and showed a numerical aberration. Although the cell line displayed an abnormal chromosome number, it was found the cell line has been shown to be pluripotent with the ability to differentiate into 3 embryonic germ layers both in vitro and in vivo. The expression of X inactive specific transcript (XIST) in mid-passage (passage 42) of undifferentiated triploid hESCs was detected, indicating X chromosome inactivation of the cell line. Moreover, when this cell line was induced to differentiate toward the trophoblast lineage, morphological and functional trophoblast cells were observed, similar to the diploid hESC line.
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