ArticleReproductive biology and endocrinology : RB&E2016
Post-thaw ATP supplementation enhances cryoprotective effect of iodixanol in rat spermatozoa.
Article in Reproductive biology and endocrinology : RB&E, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Cryopreservation of Aseel rooster sperm using different glycerol-iodixanol combinations and evaluation of post-thaw quality.BMC veterinary research · 2026Article
- Pyrroroquinoline Quinone (PQQ) Improves the Quality of Holstein Bull Semen during Cryopreservation.Animals : an open access journal from MDPI · 2024Article
- Effect of cholesterol-loaded cyclodextrin treatment on boar sperm cryopreservation.Animal bioscience · 2024Article
- Safety of Plasma Rich in Growth Factors (PRGF) as additive to healthy human sperm samples: a pilot study.JBRA assisted reproduction · 2024Article
- Overview on the Antioxidants, Egg Yolk Alternatives, and Mesenchymal Stem Cells and Derivatives Used in Canine Sperm Cryopreservation.Animals : an open access journal from MDPI · 2021Review
- Establishment of sperm cryopreservation and in vitro fertilisation protocols for rats.Scientific reports · 2020Article
- Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm.Journal of veterinary science · 2019Article
- Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundSuccessful cryopreservation of rat spermatozoa from various strains still remains a challenge. The objective of this study was to determine if combinations of OptiPrep™ (iodixanol) and adenosine 5'-triphosphate (ATP) can improve rat sperm function during the cryopreservation procedure.
methodsEpididymal rat spermatozoa were frozen under different OptiPrep™ concentrations (0, 1, 2, 3 or 4 %) and were diluted with media supplemented with or without 2 mM ATP after thawing. Post-thaw sperm motility, acrosomal membrane integrity (AMI) and mitochondrial membrane potential (MMP) were then evaluated. In addition, the effect of different OptiPrep™ concentrations on fresh and cooled rat spermatozoa was tested via motility.
resultsThere was no effect of OptiPrep™ on motility of fresh and cooled spermatozoa. The supplementation of 1 and 2 % OptiPrep™ increased motility of frozen spermatozoa at 10 min after thawing, while it did not improve motility of spermatozoa at 3 h after thawing in the absence of ATP. During incubation of thawed spermatozoa, the ATP addition protected time-dependent decrease in motility after thawing in OptiPrep™-treated samples. OptiPrep™ had no effect on AMI and MMP in frozen-thawed spermatozoa but combinations of OptiPrep™ and ATP improved MMP in frozen-thawed spermatozoa.
conclusionsIodixanol has cryoprotective effects during rat sperm freezing without any toxic effect. Moreover, the combinations of iodixanol and ATP have a beneficial role in maintaining function of frozen-thawed rat spermatozoa for long period of incubation post-thaw.
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