ArticleeLife2016
An analysis toolbox to explore mesenchymal migration heterogeneity reveals adaptive switching between distinct modes.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Transforming microfluidics for single-cell analysis with robotics and artificial intelligence.Lab on a chip · 2025Review
- Dynamic rigidity changes enable rapid cell migration on soft substrates.Nature communications · 2025Article
- 3D-printed plugs enhance cell usage efficiency for single-cell migration and neuron axon guidance assays.Cell reports methods · 2025Article
- Interpretable Fine-Grained Phenotypes of Subcellular Dynamics via Unsupervised Deep Learning.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The F-actin bundler SWAP-70 promotes tumor metastasis.Life science alliance · 2024Article
- Advances in Single-Cell Techniques for Linking Phenotypes to Genotypes.Cancer heterogeneity and plasticity · 2024Article
- Microfluidic single-cell migration chip reveals insights into the impact of extracellular matrices on cell movement.Lab on a chip · 2023Article
- An introduction to representation learning for single-cell data analysis.Cell reports methods · 2023Review
- Morphomigrational description as a new approach connecting cell's migration with its morphology.Scientific reports · 2023Article
- Multisite assessment of reproducibility in high-content cell migration imaging data.Molecular systems biology · 2023Article
- Data-driven microscopy allows for automated context-specific acquisition of high-fidelity image data.Cell reports methods · 2023Article
- Oscillatory ERK Signaling and Morphology Determine Heterogeneity of Breast Cancer Cell Chemotaxis via MEK-ERK and p38-MAPK Signaling Pathways.Bioengineering (Basel, Switzerland) · 2023Article
- Actin polymerization and depolymerization in developing vertebrates.Frontiers in physiology · 2023Review
- Article
- Local temporal Rac1-GTP nadirs and peaks restrict cell protrusions and retractions.Science advances · 2022Article
- Article
- Enhanced substrate stress relaxation promotes filopodia-mediated cell migration.Nature materials · 2021Article
- Emerging machine learning approaches to phenotyping cellular motility and morphodynamics.Physical biology · 2021Review
- ImageJ and CellProfiler: Complements in Open-Source Bioimage Analysis.Current protocols · 2021Article
- 3D mesenchymal cell migration is driven by anterior cellular contraction that generates an extracellular matrix prestrain.Developmental cell · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal (lamellipodial) migration is heterogeneous, although whether this reflects progressive variability or discrete, 'switchable' migration modalities, remains unclear. We present an analytical toolbox, based on quantitative single-cell imaging data, to interrogate this heterogeneity. Integrating supervised behavioral classification with multivariate analyses of cell motion, membrane dynamics, cell-matrix adhesion status and F-actin organization, this toolbox here enables the detection and characterization of two quantitatively distinct mesenchymal migration modes, termed 'Continuous' and 'Discontinuous'. Quantitative mode comparisons reveal differences in cell motion, spatiotemporal coordination of membrane protrusion/retraction, and how cells within each mode reorganize with changed cell speed. These modes thus represent distinctive migratory strategies. Additional analyses illuminate the macromolecular- and cellular-scale effects of molecular targeting (fibronectin, talin, ROCK), including 'adaptive switching' between Continuous (favored at high adhesion/full contraction) and Discontinuous (low adhesion/inhibited contraction) modes. Overall, this analytical toolbox now facilitates the exploration of both spontaneous and adaptive heterogeneity in mesenchymal migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.