ArticleInternational journal of molecular sciences2016
Mitochondria Related Pathway Is Essential for Polysaccharides Purified from Sparassis crispa Mediated Neuro-Protection against Glutamate-Induced Toxicity in Differentiated PC12 Cells.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.
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Who cites it
27 citing papers in PubMed, 2 syntheses or guidelines pooled it, 45 citations in OpenAlex.
- The cellular model for Alzheimer's disease research: PC12 cells.Frontiers in molecular neuroscience · 2022Pooled it
- Effects ofInternational journal of molecular sciences · 2018Pooled it
- Extracts from the Edible MushroomFoods (Basel, Switzerland) · 2026Article
- Polysaccharides with Arabinose: Key Players in Reducing Chronic Inflammation and Enhancing Immune Health in Aging.Molecules (Basel, Switzerland) · 2025Review
- Uncovering the potential of Yiqi Huoxue Jiedu formula: a promising adjunct in sepsis and septic shock management.Frontiers in cellular and infection microbiology · 2025Article
- Mulberry Leaf Polysaccharides Attenuate Oxidative Stress Injury in Peripheral Blood Leukocytes by Regulating Endoplasmic Reticulum Stress.Antioxidants (Basel, Switzerland) · 2024Article
- Moschus ameliorates glutamate-induced cellular damage by regulating autophagy and apoptosis pathway.Scientific reports · 2023Article
- Article
- The Potential of Edible and Medicinal Resource Polysaccharides for Prevention and Treatment of Neurodegenerative Diseases.Biomolecules · 2023Review
- Carboxymethylation ofOxidative medicine and cellular longevity · 2022Article
- Article
- Regulatory Effects of Damaged Renal Epithelial Cells After Repair byInternational journal of nanomedicine · 2021Article
- Ultrasonic-assisted enzymatic extraction ofRSC advances · 2020Article
- Astaxanthin Protects PC12 Cells against Homocysteine- and Glutamate-Induced Neurotoxicity.Molecules (Basel, Switzerland) · 2020Article
- Plant-Derived Antioxidants Protect the Nervous System From Aging by Inhibiting Oxidative Stress.Frontiers in aging neuroscience · 2020Review
- Protective roles of isoastilbin against Alzheimer's disease via Nrf2‑mediated antioxidation and anti‑apoptosis.International journal of molecular medicine · 2019Article
- Investigation of the neuroprotective effects of crocin via antioxidant activities in HT22 cells and in mice with Alzheimer's disease.International journal of molecular medicine · 2019Article
- Pharmacological basis for application of scutellarin in Alzheimer's disease: Antioxidation and antiapoptosis.Molecular medicine reports · 2018Article
- Genome sequence of the cauliflower mushroom Sparassis crispa (Hanabiratake) and its association with beneficial usage.Scientific reports · 2018Article
- Investigation of the neuroprotective effects of Lycium barbarum water extract in apoptotic cells and Alzheimer's disease mice.Molecular medicine reports · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study aims to explore the neuro-protective effects of purified Sparassis crispa polysaccharides against L-glutamic acid (L-Glu)-induced differentiated PC12 (DPC12) cell damages and its underlying mechanisms. The Sparassis crispa water extract was purified by a DEAE-52 cellulose anion exchange column and a Sepharose G-100 column. A fraction with a molecular weight of 75 kDa and a diameter of 88.9 nm, entitled SCWEA, was obtained. SCWEA was identified with a triple helix with (1→3)-linked Rha in the backbone, and (1→2) linkages and (1→6) linkages in the side bone. Our results indicated that the pre-treatment of DPC12 cells with SCWEA prior to L-Glu exposure effectively reversed the reduction on cell viability (by 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay) and reduced L-Glu-induced apoptosis (by Hoechst staining). SCWEA decreased the accumulation of intracellular reactive oxygen species, blocked Ca(2+) influx and prevented depolarization of the mitochondrial membrane potential in DPC12 cells. Furthermore, SCWEA normalized expression of anti-apoptotic proteins in L-Glu-explored DPC12 cells. These results suggested that SCWEA protects against L-Glu-induced neuronal apoptosis in DPC12 cells and may be a promising candidate for treatment against neurodegenerative disease.
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