Evidence map›Paper›PMID 26820741›Full record

ArticleIntervirology2015

Detection of Human Papillomavirus Infections at the Single-Cell Level.

Zhenping Shen, Xia Liu, Janice Morihara, Ayaka Hulbert, Laura A Koutsky, Nancy B Kiviat, Long Fu Xi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Intervirology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Zhenping Shen *Department of Pathology, School of Medicine, University of Washington, Seattle, WA.
Xia Liu *Department of Pathology, School of Medicine, University of Washington, Seattle, WA.
Janice Morihara *Department of Pathology, School of Medicine, University of Washington, Seattle, WA.
Ayaka HulbertDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA.
Laura A KoutskyDepartment of Epidemiology, School of Public Health, University of Washington, Seattle, WA.
Nancy B KiviatDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA.
Long Fu XiDepartment of Pathology, School of Medicine, University of Washington, Seattle, WA.
University of Washington · USChina Medical University · CN

Funding

Intratypic variation of oncogenic HPV types as a risk factor for cervical neoplasR01CA133569 · NCI · UNIVERSITY OF WASHINGTON · PI XI, LONG FU · 2009 to 2012
$1.7M
NCI NIH HHS CA133569NCI NIH HHS R01 CA133569
6 · The paper itself

Abstract

objectiveTo explore the possibility of single-cell analysis of human papillomavirus (HPV) infection.

methodsTwo hundred and twenty cells were isolated by laser capture microdissection from formalin-fixed and paraffin-embedded cervical tissue blocks from 8 women who had HPV DNA detected in their cervical swab samples. The number of type-specific HPV copies in individual cells was measured by quantitative polymerase chain reaction with and without a prior reverse transcription. The cells were assayed and counted for more than once if the corresponding swab sample was positive for ≥2 HPV types.

resultsInfection with HPV16, HPV39, HPV51, HPV52, HPV58, HPV59 and HPV73 was detected in 12 (5.5%) of 220, 3 (9.4%) of 32, 3 (5.8%) of 52, 11 (22.9%) of 48, 9 (18.8%) of 48, 3 (9.4%) of 32 and none of 20 cells, respectively. The numbers of HPV genome copies varied widely from cell to cell. The coexistence of multiple HPV types was detected in 6 (31.6%) of 19 positive cells from 1 of the 6 women who had 2 or 3 HPV types detected in their swab samples.

conclusionGiven the heterogeneity of HPV status in individual cells, further clarification of HPV infection at the single-cell level may refine our understanding of HPV-related carcinogenesis.

Indexed as

Cervix UteriDNA, ViralEpithelial CellsFemaleHumansLaser Capture MicrodissectionPapillomaviridaePapillomavirus InfectionsReal-Time Polymerase Chain ReactionSingle-Cell AnalysisDNA, Viral

Identifiers

PMID26820741
PMCPMC4748857
OpenAlexW2301632944

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.