ArticleMolecular neurobiology2017
Lentivirus-Mediated RNA Interference Targeting RhoA Slacks the Migration, Proliferation, and Myelin Formation of Schwann Cells.
Article in Molecular neurobiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 31 citations in OpenAlex.
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- MicroRNA-301a knockout attenuates peripheral nerve regeneration by delaying Wallerian degeneration.Neural regeneration research · 2026Article
- Clearing Amyloid-Beta by Astrocytes: The Role of Rho GTPases Signaling Pathways as Potential Therapeutic Targets.Brain sciences · 2024Review
- miR-301a Deficiency Attenuates the Macrophage Migration and Phagocytosis through YY1/CXCR4 Pathway.Cells · 2022Article
- Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury.Neural regeneration research · 2022Article
- Host and Clostridioides difficile-Response Modulated by Micronutrients and Glutamine: An Overview.Frontiers in nutrition · 2022Review
- SIRT6 inhibition delays peripheral nerve recovery by suppressing migration, phagocytosis and M2-polarization of macrophages.Cell & bioscience · 2021Article
- A near-infrared AIE fluorescent probe for myelin imaging: From sciatic nerve to the optically cleared brain tissue in 3D.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Macrophage-specific RhoA knockout delays Wallerian degeneration after peripheral nerve injury in mice.Journal of neuroinflammation · 2021Article
- Ascorbic acid accelerates Wallerian degeneration after peripheral nerve injury.Neural regeneration research · 2021Article
- Pannexin 1, a large-pore membrane channel, contributes to hypotonicity-induced ATP release in Schwann cells.Neural regeneration research · 2021Article
- Article
- Ascorbic Acid Facilitates Neural Regeneration After Sciatic Nerve Crush Injury.Frontiers in cellular neuroscience · 2019Article
- GSK3β inhibitor promotes myelination and mitigates muscle atrophy after peripheral nerve injury.Neural regeneration research · 2018Article
- Actin Cytoskeleton Affects Schwann Cell Migration and Peripheral Nerve Regeneration.Frontiers in physiology · 2018Article
- Inhibition of RhoA-Subfamily GTPases Suppresses Schwann Cell Proliferation Through Regulating AKT Pathway Rather Than ROCK Pathway.Frontiers in cellular neuroscience · 2018Article
- Article
- Preparation of Teased Nerve Fibers from Rat Sciatic Nerve.Bio-protocol · 2017Article
- Article
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RhoA, a member of Rho GTPases family, is known to play an important role in remodeling actin cytoskeleton. During the development of the peripheral nervous system (PNS), Schwann cells undergo proliferation, migration, and radial sorting and finally wrap the related axons compactly to form myelin sheath. All these processes involve actin cytoskeletal remodeling. However, the role of RhoA on Schwann cell during development is still unclear. To address this question, we first used a lentiviral vector-mediated short hairpin (sh) RNA targeting RhoA to knock down the expression of RhoA in the cultured Schwann cells in vitro. Effects of RhoA on Schwann cell proliferation and migration were examined by BrdU assay and transwell assay, respectively. Results of the present study indicated that downregulated RhoA expression in cultured Schwann cells significantly slacked the cells' capabilities of migration and proliferation. Then, we investigated the role of RhoA in the developing rat sciatic nerves. Immunohistology and Western blotting showed that RhoA was mainly expressed in Schwann cells in the sciatic nerves and was peaked at 2 weeks postnatal then kept in low level up to 8 weeks. In the subjected rats whose sciatic nerves were microinjected with lentiviral vectors at postnatal 3 days, we found that the lentiviruses mainly transfected Schwann cells, and the RhoA expression in the transfected Schwann cells was significantly knocked down. Four weeks after lentivirus microinjection, immunohistology and transmission electron microscopy illustrated that RhoA knockdown resulted in hypomyelination and significant decrease of the thickness of myelin in the transfected area. Overall data of current study suggested that RhoA plays a critical role in Schwann cell biology and is essential for myelination in developing peripheral nerve.
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